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. 2022 Nov 29;23(23):14947. doi: 10.3390/ijms232314947

Figure 1.

Figure 1

Viability of organotypic slice cultures (OSC) of mouse meibomian glands (mMG) over 21 days. The viability of mMG OSCs were investigated over 21 days. No decrease in viability was observed within the first 14 days of cultivation (A). Representative pictures of OSCs after live-dead staining illustrates the location and distribution of the live (green) and dead (red) cells. Bright field imaging shows the position of the mMGs within the OSC (B). The evaluation of live-dead images based on the proportion of live and dead cells and the viability of mMG during the cultivation period (C). Most of the live cells are located in the meibomian glands, while the dead cells are homogeneously distributed throughout the tissue. Data are n = 9 (A) and 4 (B,C), mean ± SEM. Significant changes compared to day 14 are assigned as */compared to day 21 are assigned as #. An arrow indicates a linear trend test. ** p ≤ 0.01, ### p ≤ 0.001, ##### p ≤ 0.0001 (C). Scale bar: 200 µm (B).