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. 2022 Nov 29;23(23):14947. doi: 10.3390/ijms232314947

Figure 5.

Figure 5

Influence of a melanocortin receptor (MCR) agonist (α-MSH) and MCR antagonist (JNJ-10229570) on lipid production in mouse meibomian gland (mMG) organotypic slice culture (OSC). RT-PCR detection of MC1R, MC3R, MC4R and MC5R mRNA in male and female mMG from three different mice (A). Representative pictures of lipid droplets visualized by Lipi Red staining of OSCs treated with medium containing up to 1000 nM α-MSH with/without 1000 nM JNJ-10229570 for 1 day (B). Lipid production increases significantly with the addition of 100 nM and 1000 nM α-MSH, whereas this effect is absent when JNJ-10229570 is added (C). Data are n = 4, mean ± SD, NC = negative control, PC = positive control (mouse brain). Scale bar: 200 µm * p ≤ 0.05, ** p ≤ 0.01.