Table 1.

Summary of the investigated characteristics of healthy and prematurely aged skin fibroblasts after phytocannabinoid treatment.

Characteristic	Young Fibroblasts		Senescent Fibroblasts
	THC	CBD	H2O2 + THC	H2O2 + CBD
Shape and size of the cell	Elongated, small	Elongated, small	Enlarged, flattened	Enlarged, flattened
Nuclear Area	↓	↔	↔	↔
Nuclear Perimeter	↓	↔	↔	↔
Nuclear Eccentricity	↑	↔	↔	↔
Nuclear circularity (roundness)	↓	↔	↔	↔
β-Gal level	↑	↔	↓	↓
BID	↔	↔↑	↔↓	↓
CB1	↔↑	↔↑	↔	↔
CB2	↔↑	↔↑	↔	↔↑
CDK2	↔↑	↔↑	↔↓	↔↓
c-Jun	↔↓	↔	↔	↔↓
Collagen (type I)	↔	↔↓	↔↑	↔↑
Collagen (type III)	↔	↔	↔	↔
Cyclin D1	↔	↔↓	↓	↓
EGFR	↓	↔	↑	↔
Elastin	↔	↔	↔↓	↓
GDF11	↔↑	↑	↔	↔↑
Hyaluronan synthase	↑	↑	↔↑	↔↑
MMP 1	↑	↔	↓	↔↓
MMP 2	↔	↔	↔↑	↔↑
MKI67	↑	↑	↔	↑
NF-κB	↔↑	↔↑	↔↓	↔↑
P16	↔	↔↑	↑	↔↑
P21	↔↓	↔↓	↔	↑
P53	↔↑	↔↑	↔	↔↓
PCNA	↔	↔	↔	↔
SIRT 1	↔↑	↔↑	↔	↔
SIRT 3, 4	↔↑	↔↑	↔↓	↔
SIRT 6	↑	↔↑	↔↓	↔
TIMP 1	↔↑	↔↑	↔↓	↔↓
Vinculin	↔↓	↓	↔	↔
Cell viability	↑	↔	↑	↑
Wound healing	↑	↑	↑	↑

Results compared to vehicle control, DMSO in healthy fibroblasts group, and H₂O₂ + DMSO. In senescent fibroblast group. ↓, Decreased/low; ↑, Increased/high; ↔, no change detected; ↔↓, tendency to decrease; ↔↑, tendency to increase.