Table 1.
Formulae and terms used by the JIP-test for the analysis of the fluorescence transient O-J-I-P.
| Formulae and Terms | Illustrations |
|---|---|
| Fo | Minimal recorded fluorescence intensity |
| Fm | Maximal recorded fluorescence intensity |
| VJ ≡ (FJ − Fo)/(Fm − Fo) | Relative variable fluorescence intensity at the J-step |
| VI ≡ (FI − Fo)/(Fm − Fo) | Relative variable fluorescence intensity at the I-step |
| dV/dto | QA maximum reduction rate |
| φPo ≡ TRo/ABS = [1 − (Fo/Fm)] | Maximum quantum yield for primary photochemistry (at t = 0) |
| ψo ≡ ETo/TRo = (1 − VJ) | Probability that a trapped exciton moves an electron into the electron transport chain beyond QA− (at t = 0) |
| φEo ≡ ETo/ABS = [1 − (Fo/Fm)]·ψo | Quantum yield for electron transport (at t = 0) |
| φDo ≡ 1 − φPo = (Fo/Fm) | Quantum yield (at t = 0) of energy dissipation |
| ABS/RC = M0 (1/VJ) (1/φPo) | Absorption flux per RC |
| TRo/RC = M0 (1/VJ) | Trapped energy flux per RC (at t = 0) |
| ETo/RC = M0 (1/VJ) ψo | Electron transport flux per RC (at t = 0) |
| DIo/RC = (ABS/RC) − (TRo/RC) | Dissipated energy flux per RC (at t = 0) |
| ABS/CSo ≈ Fo | Absorption flux per CS (at t = 0) |
| TRo/CSo = φPo·(ABS/CSo) | Trapped energy flux per CS (at t = 0) |
| ETo/CSo = φEo·(ABS/CSo) | Electron transport flux per CS (at t = 0) |
| DIo/CSo = (ABS/CSo) − (TRo/CSo) | Dissipated energy flux per CS (at t = 0) |
| Density of reaction centers | |
| RC/CSo = φPo·(VJ/Mo)·(ABS/CSo) | Density of RCs (QA-reducing PSII reaction centers) |
| PICS ≡ (RC/CSo) [φPo/(1 − φPo)] [ψo/(1 − ψo)] | Performance index on cross section basis (at t = 0) |