. 2022 Nov 25;27(23):8227. doi: 10.3390/molecules27238227

Table 4.

Inhibitory activity of the EOs on the biofilm, at 0 and 24 h.

Time 0	A. baumannii	E. coli	L. monocytogenes	P. aeruginosa	S. aureus
EG 10 µL/mL	82.48 ± 0.2 ^****	85.44 ± 0.2 ^****	85.03 ± 0.1 ^****	89.62 ± 0.2 ^****	87.17 ± 0.1 ^****
EG 20 µL/mL	92.53 ± 0.1 ^****	86.39 ± 0.2 ^****	87.56 ± 0.2 ^****	91.40 ± 0.1 ^****	93.73 ± 0.1 ^****
EH 10 µL/mL	37.53 ± 0.2 ^****	47.00 ± 0.3 ^****	77.59 ± 0.3 ^****	78.81 ± 0.2 ^****	84.23 ± 0.1 ^****
EH 20 µL/mL	74.98 ± 0.3 ^****	78.44 ± 0.4 ^****	81.06 ± 0.3 ^****	79.71 ± 0.3 ^****	84.99 ± 0.2 ^****
EP 10 µL/mL	92.08 ± 0.2 ^****	89.71 ± 0.2 ^****	79.13 ± 0.2 ^****	84.81 ± 0.1 ^****	88.51 ± 0.1 ^****
EP 20 µL/mL	92.37 ± 0.1 ^****	94.39 ± 0.1 ^****	86.65 ± 0.2 ^****	90.64 ± 0.1 ^****	95.04 ± 0.2 ^****
ELE 10 µL/mL	73.89 ± 0.3 ^****	60.58 ± 0.3 ^****	0.00 ± 0.00	64.08 ± 0.3 ^****	77.32 ± 0.2 ^****
ELE 20 µL/mL	79.61 ± 0.2 ^****	89.92 ± 0.2 ^****	92.14 ± 0.1 ^****	89.48 ± 0.1 ^****	86.03 ± 0.2 ^****
ELO 10 µL/mL	84.82 ± 0.1 ^****	85.64 ± 0.2 ^****	82.19 ± 0.3 ^****	80.91 ± 0.2 ^****	62.11 ± 0.3 ^****
ELO 20 µL/mL	86.09 ± 0.1 ^****	88.16 ± 0.1 ^****	88.14 ± 0.3 ^****	87.38 ± 0.2 ^****	73.98 ± 0.2 ^****
EV 10 µL/mL	59.39 ± 0.1 ^****	54.29 ± 0.2 ^****	0.00 ± 0.00	83.31 ± 0.3 ^****	4.36 ± 0.1 ^****
EV 20 µL/mL	63.16 ± 0.2 ^****	77.07 ± 0.2 ^****	98.67 ± 0.1 ^****	93.70 ± 0.1 ^****	58.84 ± 0.2 ^****
EW 10 µL/mL	89.15 ± 0.2 ^****	77.64 ± 0.1 ^****	0.00 ± 0.00	89.24 ± 0.2 ^****	59.16 ± 0.2 ^****
EW 20 µL/mL	89.43 ± 0.2 ^****	91.00 ± 0.1 ^****	88.78 ± 0.2 ^****	90.09 ± 0.1 ^****	75.90 ± 0.2 ^****
24 h	*A. baumannii*	*E. coli*	*L. monocytogenes*	*P. aeruginosa*	*S. aureus*
EG 10 µL/mL	67.88 ± 0.3 ^****	33.66 ± 0.2 ^****	24.31 ± 0.2 ^****	78.64 ± 0.2 ^****	71.21 ± 0.4 ^****
EG 20 µL/mL	68.39 ± 0.2 ^****	33.31 ± 0.2 ^****	35.76 ± 0.1 ^****	81.79 ± 0.3 ^****	87.15 ± 0.2 ^****
EH 10 µL/mL	9.32 ± 0.1 ^****	52.23 ± 0.2 ^****	6.10 ± 0.1 ^****	53.15 ± 0.2 ^****	73.93 ± 0.2 ^****
EH 20 µL/mL	51.64 ± 0.2 ^****	53.28 ± 0.2 ^****	25.63 ± 0.2 ^****	59.50 ± 0.1 ^****	75.65 ± 0.3 ^****
EP 10 µL/mL	52.16 ± 0.2 ^****	26.85 ± 0.2 ^****	6.18 ± 0.1 ^****	59.35 ± 0.2 ^****	82.33 ± 0.2 ^****
EP 20 µL/mL	71.31 ± 0.2 ^****	36.34 ± 0.2 ^****	67.88 ± 0.2 ^****	87.56 ± 0.3 ^****	88.84 ± 0.3 ^****
ELE 10 µL/mL	52.15 ± 0.2 ^****	62.56 ± 0.2 ^****	0.00 ± 0.00	34.58 ± 0.2 ^****	52.13 ± 0.4 ^****
ELE 20 µL/mL	70.90 ± 0.3	65.96 ± 0.2 ^****	61.19 ± 0.2 ^****	57.35 ± 0.2 ^****	58.20 ± 0.4 ^****
ELO 10 µL/mL	62.15 ± 0.3 ^****	1.77 ± 0.2 ^*	63.33 ± 0.2 ^****	52.63 ± 0.3 ^****	55.23 ± 0.3 ^****
ELO 20 µL/mL	68.94 ± 0.2 ^****	50.42 ± 0.2 ^****	65.22 ± 0.1 ^****	52.71 ± 0.2 ^****	60.41 ± 0.3 ^****
EV 10 µL/mL	14.70 ± 0.1 ^****	0.30 ± 0.2	0.00 ± 0.00	5.49 ± 0.1 ^***	50.77 ± 0.2 ^****
EV 20 µL/mL	25.29 ± 0.1 ^****	39.64 ± 0.2 ^****	4.39 ± 0.1 ^****	20.44 ± 0.1 ^****	53.49 ± 0.1 ^****
EW 10 µL/mL	30.58 ± 0.1 ^****	25.46 ± 0.2 ^****	0.00 ± 0.00	56.68 ± 0.2 ^****	94.55 ± 0.1 ^****
EW 20 µL/mL	36.92 ± 0.2 ^****	41.65 ± 0.2 ^****	57.27 ± 0.2 ^****	63.53 ± 0.1 ^****	95.83 ± 0.1 ^****

The data are the inhibition percentages calculated with respect to the untreated bacteria used as control and are reported as the mean of three independent experiments ± SD, * p < 0.05, ***p < 0.001,. ****p < 0.0001 compared with control (ANOVA followed by Dunnett’s multiple comparison test). EG = E. griffithsii, EH = E. hemiphloia, ELE = E. lesoufii, ELO = E. longicornis, EP = E. pyriformis, EV = E. viminalis, EW = E. wandoo.