Figure 11.

Clearing of a mouse heart tissue using HyClear. (a,b) Image slices of a mouse heart tissue with GFP-positive fibroblasts, using a 20× lens, at 44 µm, (a) before and (b) after tissue clearing. (c,d). MIP images of a mouse heart tissue using a 20× lens, with GFP-positive fibroblasts, (c) before and (d) after tissue clearing. 3-D reconstructed z-stack image of mouse heart before tissue clearing. (e,f) 3-D reconstructed z-stack image of mouse heart (e) before and (f) after tissue clearing. The imageable depth of the tissue increases from about 50 µm in image (e) (before) to about 155 µm in image (f) (after). The scale bars represent 100 µm.