Figure 10.

Preventive effects of resveratrol and compound 3d against H₂O₂-induced increase in intracellular ROS production: Potential role of sirtuins. Bars indicate intracellular ROS production (%) in HUVECs treated with H₂O₂ 100 µM for 2 h. In the absence of the sirtuin inhibitor sirtinol (Sirt 10 µM), both resveratrol (Resv) and 3d (30 µM) incubated for 1 h significantly protected endothelial cells from the ROS increase. Conversely, in the presence of Sirt 10 µM, the preventive effects of resveratrol and 3d against H₂O₂-induced increase in ROS were almost abolished. Data are shown as mean ± SEM. * indicates significant difference vs. vehicle (*** p < 0.001), § indicates significant difference vs. H₂O₂ 100 µM (§ p < 0.05; §§ p < 0.01), # indicates significant difference vs. Resv 30 µM + H₂O₂ 100 µM (# p < 0.05) while ^ indicates significant difference vs. 3d 30 µM + H₂O₂ 100 µM (^^ p < 0.01).