Figure 4.

The combination of S, E, and M SARS-CoV-2 recombinant proteins induced inflammatory cytokine release in human differentiated U937 macrophages and bronchial epithelial cells. (A) Differentiated U937 macrophages and (B) human bronchial epithelial cells (HBEC) from two subjects were stimulated with SARS-CoV-2 recombinant proteins at 50 ng/mL, the combination of S1 + E + M recombinant proteins at 50 ng/mL each one or with the mosaic (S-E-M) recombinant protein for 24 h. Cell supernatants were collected, and cytokines were measured by ELISA. Data are presented as scatter dot blot of n = 4 independent experiments run in triplicate, with median and interquartile range values. p-values are based on the Kruskal-Wallis test and Dunn’s post-hoc test for multiple comparison.