FIG. 8.

Immunohistochemistry for VEGF of the pouch wall treated with Rhodococcus sp. strain 4306 TDM at days 1 (A to C) and 3 (D). At day 1, VEGF-positive neutrophils (small arrows) distributed in the necrotic tissue and granulomatous tissue (indicated by arrowed bar). They were abundant in the area facing the pouch cavity (A) and oil droplets (B). (C) A higher magnification of the area indicated by a thick arrow in panel B revealed that infiltrating cells had the lobulated nuclei (arrowheads) indicative of neutrophils. At 3 days, new vessels (arrows) extended into the granulomatous tissue, where VEGF-positive cells (small arrows) were abundant. At a higher magnification, most of the VEGF-positive cells were seen to have a small, round shape with the lobulated nuclei indicative of neutrophils (upper inset); a few positive cells were larger and had the kidney-shaped nuclei indicative of macrophages (lower inset). M, muscular layer; N, necrotic tissue; O, oil droplets. (A and B) Bar, 20 μm. (C) Bar, 5 μm. (D) Bar, 20 μm. (Insets) Bar, 5 μm.