Figure 4.

Involvement of Lonp1 during restoration induced by HPPSC_EVs. Scramble and siRNA targeting Lonp1 were transfected into R28 cells. After incubation, Lonp1 deficient cells were exposed to CoCl₂ (200 μM). After incubation for 9 h, the hypoxia-induced cells were treated with HPPSC_EVs. Then, Western blot analyses were performed. The results are expressed as a mean ± SEM. Statistical significance was determined using a nonparametric statistical test, followed by the Mann–Whitney U test and an unpaired t test (^# p < 0.05,^## p < 0.01 vs. control with scramble, * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001). All experiments were performed in triplicate. The KD experiments were performed five times.