Figure 5.
Intrinsic fluorescence spectra of C-TERb (A–D) and C-TERa (E,F) after 0 h (solid line), 24 h (dashed line), 48 h (dotted line), 72 h (dashed-dotted line), and 96 h (dashed-double-dotted line) of incubation in TBS at ~20 °C (CC-TER = 10 µM). (A) ITF and (B) normalised first ITF band spectra (λex = 295 nm, sw = 10 nm). (C,E) ITyrF and (D,F) normalised first ITyrF band spectra (λex = 275 nm, sw = 10 nm). On each normalised spectrum, the associated difference spectrum is determined between 0 and 96 h (diamonds).