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Binding of biotinylated, full-length rgC1qR (MF) to immobilized protein A. Biotinylated rgC1qR (∼2 μg/ml) was incubated (60 min, 37°C) on protein A-coated microtiter wells. Binding was detected with AP-STRAV and pNPP substrate. C1q- and BSA-coated microtiter wells served as controls. Values represent means ± SD; n = 3. O.D., optical density.
