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. 2022 Nov 29;14(23):5909. doi: 10.3390/cancers14235909

Figure 2.

Figure 2

Depiction of the extent to which AM signaling is expressed and regulated in melanoma cells. (A) AM and receptors expressed in melanoma cells depicted using immunofluorescence of A375, SK-MEL-28, and MeWo cells stained with antibodies against AM, CLR, RAMP2, and RAMP3, revealing localization in the cytoplasm. Negative control for immunostaining was achieved with IgG-control. (B) AM expression induced by a hypoxia mimetic in melanoma cells. Total RNA (1 μg, DNA free) prepared from MeWo, SK-MEL-28, and A375 cells were reverse transcribed into cDNA under normoxic or hypoxic conditions and relative AM mRNA was estimated using a real-time quantitative reverse transcriptase polymerase chain reaction. There were significant differences between cells treated with desferrioxamine mesylate (DFX) and untreated control cells in terms of AM expression: * p < 0.05; ** p < 0.01; *** p < 0.001. Each experiment is representative of five independent experiments. Results are shown as means ± SD.