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. 2022 Nov 26;23(23):14809. doi: 10.3390/ijms232314809

Figure 4.

Figure 4

The quantity of TUNEL-positive photoreceptor nuclei was increased in pomt2 mutant retinas. A TUNEL assay was carried out on zebrafish retinal sections at 6-mpf (green fluorescence). The sections were counter-stained with DAPI (blue fluorescence). (A) Wild-type retina. (B) pomt2 mutant retina. Note examples of TUNEL-positive cone nuclei. (C) pomt2 mutant retina. Note examples of TUNEL-positive rod nuclei. (D) Quantification of TUNEL-positive rod nuclei (n = 4). The number of TUNEL-positive rod nuclei was significantly increased in pomt2 mutant retinas compared to the controls. (E) Quantification of TUNEL-positive cone nuclei (n = 4). The number of TUNEL-positive cone nuclei was significantly increased in pomt2 mutant retinas compared to the controls. Scale bar in C: 30 µm.