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The purification balance sheet of anti-MRSA substance (P1) from SPR-20. The antimicrobial activity was determined by an agar well diffusion test against MRSA isolate 2468.
| Purification Step | Volume (mL) | Total Protein (mg) | Total Activity (AU) | Specific Activity (AU/mg) | Purification Factor |
%Yield |
|---|---|---|---|---|---|---|
| Cell-free supernatant | 1740 | 446 | 69,600 | 156 | 1 | 100 |
| Ammonium sulfate precipitation |
205 | 231 | 65,600 | 284 | 2 | 94 |
| CIEX | 277 | 49 | 22,160 | 452 | 3 | 32 |
| RPC (P1) | 23 | 9 | 14,720 | 1694 | 11 | 21 |
