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. 2022 Nov 29;14(23):5900. doi: 10.3390/cancers14235900

Figure 3.

Figure 3

Knockdown of p300 and CBP inhibits AR signaling. (A) Analysis of p300, CBP, and PSA protein expression in LNCaP cells carrying the separate or combined p300 and CBP knockdowns. Forty-eight hours after siRNA transfection, LNCaP cells were placed in medium supplemented with charcoal-stripped serum for 24 h followed by stimulation with R1881 (1 nM) for 18 h. Protein expression was examined using Western blot analysis. (B) PSA gene expression was examined in LNCaP cells carrying the separate or combined p300 and CBP knockdowns. Forty-eight hours after siRNA transfection, LNCaP cells were placed in medium supplemented with charcoal-stripped serum for 24 h followed by stimulation with R1881 (1nM) for 6 h. Gene expression was assayed by qRT-PCR. 18S gene was used for normalization. Results are expressed as the mean (n = 3) ± SD. * p < 0.01; *** p < 0.0001; ns—non-significant. Full size blots of are shown in Figure S4.