Figure 5.

ACLY inhibition results in the suppression of ligand-dependent and -independent PSA expression. (A) Western blot analysis of the AR expression in LNCaP, C4-2B, and 22Rv1 PC cells. (B) LNCaP, C4-2B, and 22Rv1 cells were cultured under androgen-depleted conditions for 24 h followed by treated with BMS 303141 (BMS) (30 μM), enzalutamide (Enz) (2.5 μM), and R1881 (1 nM) in for 18 hrs. Expression of PSA was examined in the cell culture supernatants using PSA ELISA kit. Columns, means of three different experiments; bars, SDs. ** p < 0.001; *** p < 0.0001. Full size blots of are shown in Figure S4.