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. 2022 Nov 25;11(23):3770. doi: 10.3390/cells11233770

Figure 8.

Figure 8

HSP90 client proteins and ERS (ER stress) marker protein expression in UBA52 overexpressed SH-SY5Y cells. (a) SH-SY5Y cells were transfected with PcDNA3.1 or Myc-UBA52 with or without rotenone treatment, and protein expression of client proteins of HSP90 was immunoblotted with indicated antibodies; nexp= 3. (b) Blots represent the protein expression of ER stress markers in PcDNA3.1 or Myc-UBA52 transfected SH-SY5Y cells with or without rotenone or tunicamycin (Tm) treatment. Graphs indicate the statistical analysis of various proteins normalized against β-Actin; nexp = 3. Quantifications are the mean and SEM of at least three independent experiments, and statistical analyses were performed using two-way ANOVA, followed by Tukey’s multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001 control vs. Rotenone; # p < 0.05, ## p < 0.01, ### p < 0.001 Myc-UBA52 vs. Rotenone; $$ p < 0.01 control vs. Myc-UBA52.