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. 2022 Nov 25;14(23):5025. doi: 10.3390/nu14235025

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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PLR attenuated oxidative stress and upregulated the placental Nrf2/HO-1/NQO1 pathway in PE mice. (A–D) ELISA was used to analyze the concentrations of malondialdehyde (MDA) and glutathione (GSH) in the placenta and serum of different groups. (E) Representative Western blot bands and the relative density analysis results of Nrf2, HO-1, and NQO1 in placenta. β-actin was set as a loading control, and the relative expressions were normalized to the control. (F–I) Relative mRNA levels of Sod1, Nrf2, Ho-1, and Nqo1 in placenta. For (A–D), n = 8 for each group. For (E), n = 3 for each group. For (F–I), n = 6 for each group. Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 (ANOVA test).