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. 2000 Apr;68(4):2096–2101. doi: 10.1128/iai.68.4.2096-2101.2000

TABLE 1.

Summary of BLAST search of clones that were specific for E. coli strain C5 and had significant homologiesa

Cloneb Insert size (bp) Sequence(s) with similarityc Score Probability GenBank accession no.
SauE15.A1 163 IS629 (N), plasmid pO157, E. coli O157:H7 315 e−84 AB011549
SauE15.A7 190 iroC (N), ATP cassette transporter (iron-regulated locus), Salmonella enterica serovar Typhi 157 e−37 U6129
SauE15.B2 294 repB (N); replication protein, plasmid pCD1; Yersinia pestis 123 e−26 AF053946
SauE15.B6 157 kps (N), promoter region of polysialic acid gene cluster region 3, E. coli 242 e−62 U05251
SauE15.B9 107 traD (N), F sex factor plasmid, E. coli 198 e−50 M29254
SauE15.B10 240 ORF 34 and 35 (P), 102-kb unstable region, Y. pestis 69 e−12 CAA21357
SauE15.B12 479 unknown protein (P), E. coli ec11 102 e−21 AF044503
SauE15.C1 100 r6 (N), transposase, pathogenicity island of E. coli CFT073 198 e−49 AF081285
SauE15.C6 119 IS100 (N), Y. pestis 228 e−58 L19030
SauE15.C7 155 TonB-dependent receptor HI1217 precursor (P), Haemophilus influenzae 52 e−7 P45114
SauE15.C9 273 rhuM (N), pathogenicity island of Salmonella enterica serovar Typhimurium (SPI3) 311 e−83 AF106566
SauE15.C11 77 orfE (N); promoter-distal region of the tra operon, plasmid R100; S. flexneri 129 e−29 X55815
SauE15.D4 153 IS100 (N), Y. pestis 287 e−76 L19030
SauE15.D8 347 r3 (N), beta-cystathionase, pathogenicity island of E. coli CFT073 615 e−174 AF081286
SauE15.E4 281 senB (N), enterotoxin, E. coli 541 e−152 Z54195
SauE15.E11 314 traJ, Y (N), plasmid R1–19, E. coli 523 e−147 M19710
SauE15.F3 422 chuA (P), heme utilization gene, E. coli O157:H7 98 e−20 U67920
SauE15.F9 137 Thioesterase (P), Bacillus sp. 48 e−6 AB016427
SauE15.F10 210 r3 (N), beta-cystathionase, pathogenicity island of E. coli CFT073 408 e−112 AF081286
SauE15.G3 206 traG (N), plasmid R100, S. flexneri 165 e−39 U01159
SauE15.G6 328 IS100 (N), Y. pestis 480 e−134 L19030
SauE15.H5 200 HMWP1 protein (P), Yersinia enterocolitica 80 e−15 CAA73127
SauE15.H7 150 Oxydoreductase (P), Thermotoga maritima 160 e−11 AE001762
SauE15.H10 141 traT (N), plasmid R100, E. coli 280 e−74 J01769
SauE15.H11 160 Hemoglobin protease (P), E. coli EB1 50 e−6 CAA11507
SauE15.I3 176 asst (N), arylsulfate sulfotransferase, Klebsiella sp. 341 e−92 U32616
SauE15.I11 162 chuA (N), heme utilization gene, E. coli O157:H7 305 e−82 U67920
SauE15.J7 118 iroB (N), glucosyl transferase homolog, Salmonella serovar Typhi 74 e−12 U62129
SauE15.J9 96 IS100 (N), Y. pestis 174 e−42 L19030
SauE15.M4 193 r3 and malX (N), pathogenicity island of E. coli CFT073 383 e−104 AF081286
SauE15.M8 149 Delta-(l-α-aminoadipyl)-l-cyteinyl-d-valine synthetase (P), Penicillium sp. 65 e−11 P26046
SauE15.M12 119 senB (N), enterotoxin of enteroinvasive E. coli 228 e−58 Z54195
SauE15.N7 188 Plasmid pColBM-C1139 (N), E. coli 208 e−52 M35683
SauE4.A2 321 ORF 36 (N), 102-kb unstable region, Y. pestis 135 e−30 AL031866
SauE4.A5 249 r3 (N), beta-cystathionase, pathogenicity island of E. coli CTF073 355 e−96 AF081286
SauE4.B4 360 IS200 (N), E. coli 523 e−147 L25845
SauE4.C7 275 Hippurate hydrolase (P), Campylobacter jejuni 54 e−7 P45493
SauE4.C11 255 Pristinamycine I synthase (P), Streptomyces spp. 51 e−6 CAA67248
SauE4.D3 239 hlyB (N), hemolysine, E. coli 474 e−132 M81823
SauE4.E3 263 shuX genes (N), heme utilization genes, Shigella dysenteriae 387 e−106 U64516
SauE4.E11 242 IS66 (N), E. coli 329 e−88 AF119170
SauE4.F8 188 sorC genes (N), sor operon for l-sorbose utilization, Klebsiella pneumoniae 139 e−31 X66059
SauE4.F9 439 YfkN (P), Bacillus subtilis 57 e−8 BAA23404
SauE4.F12 324 kpsM (N), polysialic acid gene cluster region 3, E. coli K1 642 0 M57382
SauE4.H2 85 sorM (N), sor operon for l-sorbose utilization, K. pneumoniae 105 e−22 X66059
SauE4.I2 431 yihA (N), plasmid R100, S. flexneri 829 0 AP000342
TspE4.A5 271 pap and prsK (N), P-pili protein, E. coli 498 e−139 X61239
TspE4.A8 216 ORF 17 kD of prs pili operon (N), cytoplasmic protein, E. coli 387 e−106 X61238
TspE4.A9 179 kpsT (N), polysialic acid gene cluster region 3, E. coli K1 347 e−94 M57381
TspE4.A10 212 HecB (P), putative hemolysin activator transporter, Erwinia chrysanthemi 73 e−13 AAC31980
TspE4.B1 229 r1 (N), pathogenicity island of E. coli CFT073 430 e−119 AF081286
TspE4.B5 215 Sensory transduction histidine kinase (P), Synechocystis sp. 52 e−7 BAA18223
TspE4.B9 319 senB (N), enterotoxin of enteroinvasive E. coli 617 e−175 Z54195
TspE4.B12 430 IS100 (N), Y. pestis 698 0 L19030
TspE4.C10 267 Intergenic K42 capsule cluster (N), E. coli 466 e−129 AF118251
TspE4.D2 232 waaL (N), lipid A core-to-surface polymer ligase, E. coli 404 e−111 AF019746
TspE4.D4 245 ORF 169 (N), plasmid F, E. coli 456 e−126 X17539
TspE4.D10 222 cnf1 (N), cytotoxic necrotizing factor, E. coli 440 e−122 X70670
TspE4.D11 217 hlyB (N), hemolysin, E. coli 422 e−117 M81823
TspE4.E3 298 hlyD (N), hemolysin, E. coli 553 e−156 M10133
TspE4.E4 267 ORF 95 (N), plasmid F, E. coli 482 e−134 X17539
TspE4.E6 190 l-sorbose P reductase (P), K. pneumoniae 112 e−25 P37084
TspE4.E8 285 hlyB (N), hemolysin, E. coli 541 e−152 M81823
TspE4.G7 238 tra (N), plasmid F, E. coli 448 e−124 X61575
TspE4.G8 323 Transmembrane protein (P), E. coli 82 e−15 AAA92620
TspE4.H1 283 Arginine deiminase (P), Pseudomonas aeruginosa 63 e−10 P13981
TspE4.H9 179 traT (N), plasmid R100, E. coli 353 e−96 J01769
TspE4.H10 223 prf and papI (N), adhesin regulatory gene, E. coli 418 e−115 X76613
TspE4.H11 279 ORF 9 (N), plasmid F, E. coli 456 e−127 X17539
TspE4.I10 269 neuC (N), capsule gene cluster, E. coli 492 e−137 M84026
TspE4.J1 327 yhtA (N), plasmid R100, E. coli 521 e−146 AP000342
TspE4.J6 221 chuA (N), heme utilization gene, E. coli O157:H7 375 e−102 U67920
TspE4.K3 180 iss (N), serum survival, E. coli 270 e−70 AF042279
TspE4.K8 184 IS100 (N), E. coli 190 e−47 L19030
prf and papB (N), E. coli 143 e−32 X76613
TspE15.A1 332 Na+ H+ antiporter (P), H. influenzae 96 e−20 Q57007
TspE15.C1 299 hra (N), heat-resistant agglutinin, E. coli 99 537 e−151 U07174
TspE15.C3 386 hcp (N), E. coli 81 e−14 AF044503
TspE15.D7 239 Protein STBA (P), plasmid NR1, E. coli 87 e−17 P11904
TspE15.D9 230 chuA (P), heme utilization gene, E. coli O157:H7 89 e−18 AAC44857
TspE15.E7 360 kpsS (N), capsule gene cluster region 1, E. coli K5 531 e−149 X74567
TspE15.G12 287 Putative amino transferase (P), B. subtilis 72 e−12 Q08432
TspE15.H2 258 Pyruvate formate lyase-activating enzyme (P), Streptococcus mutans 51 e−6 AAB89799
TspE15.H5 310 cnf1 (N), cytotoxic necrotizing factor, E. coli 601 e−170 U42629
TspE15.H9 273 Major fimbrial subunit of F17-like fimbriae (P), E. coli 48 e−5 I41206
TspE15.I2 112 prs and papE (N), P-pili protein, E. coli 222 e−57 X62158
a

Only homologies with at least probability of e−5 were retained. Homologies to bacteriophages (n = 21) are not shown. 

b

Clones are designated by the name of the enzyme (Sau or Tsp), followed by the name of the strain used for the subtraction (E4 or E15) and a code composed of a letter and a number. 

c

The name of the gene sequence is given (with similarity type in parentheses), followed by the product or function that is encoded by the gene and/or the gene's location, as well as the organism name. N, similarity at the nucleotide level; P, similarity at the protein level; ORF, open reading frame.