Table 3.
Quantified phenolic compounds in the analyzed smoothies by HPLC-ESI-TOF-MS in µg/mL expressed as average ± standard deviation.
| Phenolic Compound |
S1 | S2 | S3 | S4 | S5 | S6 | S7 | S8 | S9 | S10 | S11 | S12 | S13 | S14 | S15 | S16 | S17 | S18 | S19 | S20 | S21 | S22 | S23 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gallic acid | 367.25 ± 0.53 | <LOQ | 201.97 ± 0.36 | 5.56 ± 0.16 | 42.50 ± 0.08 | 32.47 ± 0.06 | 570.66 ± 0.74 | 62.27 ± 0.22 | 226.90 ± 0.39 | 964.22 ± 1.15 | 157.69 ± 0.32 | 405.54 ± 0.57 | 34.27 ± 0.07 | 41.18 ± 0.08 | 679.61 ± 0.85 | <LOQ | 31.44 ± 0.06 | 207.83 ± 0.37 | 40.15 ± 0.20 | 17.98 ± 0.04 | 543.29 ± 0.71 | 19.69 ± 0.05 | <LOQ |
| Chlorogenic acid isomer a | 532.92 ± 1.21 | 791.93 ± 1.48 | 1163.81 ± 1.86 | 673.74 ± 1.35 | <LOQ | <LOQ | 170.39 ± 0.84 | 230.75 ± 0.90 | 181.16 ± 0.85 | 500.54 ± 1.18 | 94.49 ± 0.76 | 160.96±0.51 | 697.92 ± 1.38 | 168.30 ± 0.53 | 1117.11 ± 1.81 | 859.87 ± 1.54 | 1226.82 ± 1.92 | 732.40 ± 1.41 | 713.90 ± 1.40 | <LOQ | 116.85 ± 0.79 | 185.17 ± 0.58 | 1144.12 ± 1.84 |
| Chlorogenic acid isomer b | 1232.31 ± 1.93 | 926.29 ± 1.61 | 784.19 ± 1.47 | 1015.41 ± 1.70 | <LOQ | <LOQ | 741.19 ± 1.42 | 581.39 ± 1.26 | 448.95 ± 1.12 | 999.52 ± 1.69 | 615.80 ± 1.30 | 354.76±1.03 | 1194.18 ± 1.89 | 318.21 ± 0.99 | 1090.78 ± 1.78 | 1052.55 ± 1.74 | 1353.11 ± 2.05 | 1189.69 ± 1.88 | 946.19 ± 1.63 | <LOQ | 409.35 ± 1.08 | 112.41 ± 0.78 | 1363.95 ± 2.06 |
| Chlorogenic acid isomer c | 45.73 ± 0.71 | 111.33 ± 0.36 | 523.06 ± 1.20 | 139.81 ± 0.45 | <LOQ | <LOQ | 81.93 ± 0.27 | 97.39 ± 0.32 | 105.24 ± 0.34 | 167.15 ± 0.53 | 106.08 ± 0.35 | 79.30±0.27 | 155.04 ± 0.49 | 39.27 ± 0.14 | 577.79 ± 1.26 | 95.03 ± 0.76 | 316.56 ± 0.99 | 166.47 ± 0.53 | 185.91 ± 0.59 | <LOQ | 80.20 ± 0.27 | 57.34 ± 0.20 | 139.74 ± 0.81 |
| Coumaroylquinic acid isomer a | 133.26 ± 0.43 | 145.68 ± 0.47 | 186.47 ± 0.59 | 122.63 ± 0.40 | <LOQ | <LOQ | 80.11 ± 0.27 | 37.49 ± 0.14 | 21.63 ± 0.09 | 65.14 ± 0.22 | 34.90 ± 0.13 | 115.07±0.37 | 136.37 ± 0.44 | 129.03 ± 0.42 | 1006.93 ± 1.69 | 187.89 ± 0.59 | 501.91 ± 1.18 | 139.30 ± 0.45 | 98.80 ± 0.32 | <LOQ | 59.14 ± 0.20 | 36.70 ± 0.14 | 204.54 ± 0.64 |
| Coumaroylquinic acid isomer a | 147.81 ± 0.47 | 157.45 ± 0.50 | 182.93 ± 0.58 | 131.98 ± 0.42 | <LOQ | <LOQ | 93.75 ± 0.31 | 35.58 ± 0.13 | 27.66 ± 0.11 | 59.72 ± 0.21 | 36.15 ± 0.14 | 141.90±0.45 | 200.41 ± 0.63 | 137.21 ± 0.44 | 1023.10 ± 1.71 | 189.33 ± 0.60 | 728.71 ± 1.41 | 154.30 ± 0.49 | 105.86 ± 0.35 | <LOQ | 81.45 ± 0.27 | 46.78 ± 0.17 | 199.41 ± 0.63 |
| Protocatehuic acid isomer a | 16.50 ± 0.04 | 12.11 ± 0.03 | 8.04 ± 0.16 | 392.01 ± 0.56 | 40.53 ± 0.08 | <LOQ | 16.13 ± 0.04 | 13.99 ± 0.04 | 0.08 ± 0.02 | 53.97 ± 0.10 | 95.67 ± 0.25 | 28.36±0.06 | 75.46 ± 0.23 | 4.62 ± 0.02 | 66.21 ± 0.12 | 33.88 ± 0.07 | 32.68 ± 0.07 | 25.71 ± 0.05 | 13.95 ± 0.04 | <LOQ | 26.49 ± 0.06 | 83.11 ± 0.14 | 12.14 ± 0.03 |
| Protocatehuic acid isomer b | <LOQ | <LOQ | 71.94 ± 0.12 | 688.17 ± 0.86 | 55.03 ± 0.10 | <LOQ | <LOQ | 128.77 ± 0.29 | <LOQ | <LOQ | 35.90 ± 0.07 | <LOQ | 49.74 ± 0.21 | <LOQ | <LOQ | <LOQ | 4.29 ± 0.02 | <LOQ | <LOQ | <LOQ | <LOQ | 95.95 ± 0.25 | <LOQ |
| Catechin | 138.09 ± 0.24 | 98.49 ± 0.13 | 165.39 ± 0.32 | 155.18 ± 0.29 | 440.95 ± 1.10 | <LOQ | 94.42 ± 0.12 | 490.36 ± 1.24 | 82.07 ± 0.09 | 128.59 ± 0.22 | 236.22 ± 0.52 | 68.69±0.05 | 100.04 ± 0.14 | 60.64 ± 0.03 | 172.85 ± 0.34 | <LOQ | 227.13 ± 0.50 | 177.63 ± 0.36 | 146.01 ± 0.27 | <LOQ | 88.55 ± 0.10 | 338.05 ± 0.81 | 150.17 ± 0.28 |
| Epicatechin | 412.15 ± 1.02 | 458.72 ± 1.15 | 338.92 ± 0.81 | 485.87 ± 1.23 | 81.51 ± 0.08 | <LOQ | 280.50 ± 0.65 | 408.96 ± 1.01 | 258.08 ± 0.58 | 370.42 ± 0.90 | 199.85 ± 0.42 | 91.06±0.11 | 105.29 ± 0.15 | 80.31 ± 0.08 | 417.27 ± 1.03 | 54.91 ± 0.01 | 222.64 ± 0.48 | 403.77 ± 1.00 | 498.76 ± 1.26 | <LOQ | 211.96 ± 0.45 | 267.64 ± 0.61 | 591.24 ± 1.53 |
| Phloridzin | 340.25 ± 1.02 | 685.88 ± 1.72 | 596.35 ± 1.54 | 365.08 ± 1.07 | 33.94 ± 0.07 | <LOQ | 20.00 ± 0.38 | 8.71 ± 0.35 | 50.23 ± 0.44 | 211.09 ± 0.76 | 100.02 ± 0.54 | 142.84±0.62 | 287.98 ± 0.92 | 80.44 ± 0.50 | 777.90 ± 1.91 | 687.57 ± 1.72 | 601.08 ± 1.55 | 304.90 ± 0.95 | 602.91 ± 1.55 | <LOQ | 54.16 ± 0.45 | 67.70 ± 0.27 | 504.35 ± 1.35 |
| Phloretin 2′-xyloglucoside | 36.34 ± 0.41 | 124.84 ± 0.59 | 55.60 ± 0.45 | 80.80 ± 0.31 | <LOQ | <LOQ | 48.35 ± 0.21 | 14.03 ± 0.12 | 32.17 ± 0.07 | 57.51 ± 0.16 | 7.16 ± 0.10 | 72.05±0.28 | 66.72 ± 0.27 | 37.06 ± 0.18 | 395.60 ± 1.14 | 116.66 ± 0.57 | 239.00 ± 0.82 | 78.62 ± 0.30 | 82.84 ± 0.31 | <LOQ | 30.77 ± 0.16 | 11.02 ± 0.11 | 102.55 ± 0.54 |
| Narirutin | 60.51 ± 0.25 | 32.44 ± 0.07 | 4.00 ± 0.09 | 7.12 ± 0.10 | <LOQ | <LOQ | 23.88 ± 0.04 | 18.94 ± 0.13 | 17.19 ± 0.01 | 0.72 ± 0.08 | 19.48 ± 0.02 | 16.25±0.01 | 22.43 ± 0.03 | 16.34 ± 0.01 | 37.25 ± 0.18 | 20.69 ± 0.02 | 31.65 ± 0.06 | 32.92 ± 0.07 | 32.12 ± 0.07 | <LOQ | 19.01 ± 0.02 | <LOQ | 19.21 ± 0.13 |
| Naringin | 63.75 ± 0.26 | 15.35 ± 0.01 | <LOQ | 18.90 ± 0.13 | 56.77 ± 0.45 | 19.39 ± 0.02 | 84.15 ± 0.51 | <LOQ | <LOQ | 47.25 ± 0.43 | 37.41 ± 0.18 | 147.01±0.63 | 28.06 ± 0.39 | 41.11 ± 0.42 | <LOQ | <LOQ | <LOQ | 124.72 ± 0.59 | <LOQ | <LOQ | 28.74 ± 0.39 | 15.45 ± 0.12 | <LOQ |
| Hesperidin | 206.93 ± 0.75 | 30.43 ± 0.06 | <LOQ | 53.01 ± 0.23 | 314.24 ± 0.97 | 28.60 ± 0.16 | 508.80 ± 1.36 | <LOQ | <LOQ | 332.79 ± 1.01 | 178.31 ± 0.70 | 554.81±1.46 | 305.55 ± 0.95 | 216.93 ± 0.77 | <LOQ | 27.08 ± 0.05 | <LOQ | 325.76 ± 0.99 | <LOQ | 31.24 ± 0.06 | 445.35 ± 1.24 | 77.77 ± 0.49 | 27.66 ± 0.05 |
| Naringenin | <LOQ | 19.28 ± 0.02 | <LOQ | 17.83 ± 0.01 | 16.77 ± 0.01 | <LOQ | <LOQ | 17.88 ± 0.01 | <LOQ | <LOQ | 21.46 ± 0.03 | <LOQ | 22.65 ± 0.03 | 22.41 ± 0.38 | <LOQ | <LOQ | <LOQ | 16.70 ± 0.01 | <LOQ | <LOQ | 17.15 ± 0.01 | 18.73 ± 0.02 | <LOQ |
| Hesperetin | <LOQ | 6.84 ± 0.10 | <LOQ | <LOQ | 16.78 ± 0.01 | <LOQ | 16.58 ± 0.01 | <LOQ | <LOQ | <LOQ | 15.57 ± 0.01 | <LOQ | 16.66 ± 0.01 | 492.79 ± 1.33 | <LOQ | <LOQ | <LOQ | <LOQ | <LOQ | <LOQ | 22.33 ± 0.03 | 16.34 ± 0.01 | <LOQ |
| Didymin | 57.14 ± 0.24 | 16.15 ± 0.01 | <LOQ | 1.41 ± 0.08 | 77.46 ± 0.30 | 18.08 ± 0.02 | 9.57 ± 0.36 | <LOQ | <LOQ | 28.22 ± 0.39 | 38.72 ± 0.19 | 26.27 ± 0.39 | 79.45 ± 0.30 | 80.07 ± 0.30 | <LOQ | <LOQ | <LOQ | 36.92 ± 0.41 | <LOQ | <LOQ | 2.50 ± 0.34 | 23.30 ± 0.14 | <LOQ |
| Quercetin 3-rhamnoside | 44.12 ± 0.43 | 234.36 ± 0.81 | 254.54 ± 0.85 | 122.60 ± 0.58 | <LOQ | <LOQ | 28.29 ± 0.16 | 11.14 ± 0.36 | 55.57 ± 0.23 | 25.54 ± 0.39 | 51.11 ± 0.22 | 26.86 ± 0.15 | 163.30 ± 0.67 | 67.58 ± 0.27 | 32.10 ± 0.40 | 84.19 ± 0.51 | 64.06 ± 0.26 | 74.14 ± 0.29 | 69.41 ± 0.48 | <LOQ | 26.95 ± 0.15 | 6.24 ± 0.09 | 59.61 ± 0.46 |
| Quercetin dihydrate isomer a | 18.52 ± 0.02 | 132.41 ± 0.60 | <LOQ | 19.32 ± 0.02 | 18.99 ± 0.02 | 13.29 ± 0.36 | 20.32 ± 0.02 | <LOQ | 15.36 ± 0.12 | 21.32 ± 0.03 | 15.97 ± 0.01 | 18.47 ± 0.02 | 19.38 ± 0.02 | 83.39 ± 0.50 | <LOQ | <LOQ | <LOQ | 20.12 ± 0.02 | 20.87 ± 0.03 | 36.51 ± 0.18 | 20.21 ± 0.02 | <LOQ | 47.17 ± 0.21 |
| Quercetin 3-O-beta-D-xylopyranoside | 56.29 ± 0.24 | 128.42 ± 0.60 | 171.28 ± 0.68 | 27.76 ± 0.39 | <LOQ | <LOQ | 28.82 ± 0.05 | 47.82 ± 0.21 | 29.40 ± 0.06 | 72.35 ± 0.21 | 20.07 ± 0.13 | 1.02 ± 0.08 | 44.44 ± 0.43 | 38.18 ± 0.18 | 13.94 ± 0.36 | 32.19 ± 0.17 | 81.85 ± 0.31 | 30.54 ± 0.16 | 86.18 ± 0.32 | <LOQ | 4.79 ± 0.09 | 33.83 ± 0.07 | 55.70 ± 0.23 |
| Isoquercetin | 30.88 ± 0.16 | 43.91 ± 0.42 | 392.84 ± 1.13 | 230.14 ± 0.80 | <LOQ | <LOQ | 20.98 ± 0.03 | 24.86 ± 0.04 | 31.90 ± 0.06 | 23.73 ± 0.14 | 15.68 ± 0.12 | 25.09 ± 0.04 | 120.26 ± 0.58 | 32.66 ± 0.07 | 33.30 ± 0.17 | 20.04 ± 0.13 | 50.82 ± 0.22 | 32.54 ± 0.07 | 39.32 ± 0.19 | <LOQ | 22.94 ± 0.03 | 33.71 ± 0.07 | 29.85 ± 0.16 |
| Hyperoside | 9.83 ± 0.10 | 43.32 ± 0.20 | 45.17 ± 0.43 | 18.91 ± 0.37 | 19.52 ± 0.13 | <LOQ | 22.11 ± 0.03 | 29.26 ± 0.16 | 22.93 ± 0.03 | 10.81 ± 0.11 | 52.70 ± 0.23 | 26.81 ± 0.05 | 115.12 ± 0.57 | 29.55 ± 0.06 | 26.45 ± 0.15 | 6.29 ± 0.09 | 38.57 ± 0.19 | 29.79 ± 0.06 | 11.91 ± 0.11 | <LOQ | 25.01 ± 0.04 | 64.15 ± 0.26 | 41.04 ± 0.19 |
| Kaempferol-3-glucoside | <LOQ | 18.38 ± 0.02 | 18.52 ± 0.13 | 25.60 ± 0.04 | 18.44 ± 0.02 | <LOQ | <LOQ | 72.85 ± 0.48 | <LOQ | 15.46 ± 0.01 | 32.47 ± 0.17 | <LOQ | 58.98 ± 0.24 | 19.29 ± 0.02 | 17.60 ± 0.01 | <LOQ | 31.91 ± 0.17 | <LOQ | 16.91 ± 0.01 | <LOQ | <LOQ | 58.21 ± 0.45 | 13.67 ± 0.11 |
| Kaempferol-3-glucuronide | <LOQ | <LOQ | <LOQ | <LOQ | 39.13 ± 0.41 | <LOQ | <LOQ | 89.21 ± 0.52 | <LOQ | <LOQ | 14.27 ± 0.12 | <LOQ | <LOQ | <LOQ | <LOQ | <LOQ | 38.55 ± 0.19 | <LOQ | <LOQ | <LOQ | <LOQ | 76.44 ± 0.29 | <LOQ |
| Kaempferol 3-(6-acetylgalactoside) isomer a | <LOQ | <LOQ | <LOQ | <LOQ | 54.85 ± 0.23 | <LOQ | <LOQ | 85.64 ± 0.51 | <LOQ | <LOQ | 25.44 ± 0.04 | <LOQ | 17.47 ± 0.01 | <LOQ | <LOQ | <LOQ | 32.62 ± 0.07 | <LOQ | <LOQ | <LOQ | <LOQ | 43.47 ± 0.20 | 17.21 ± 0.01 |
| Kaempferol 3-(6-acetylgalactoside) isomer b | <LOQ | <LOQ | 15.62 ± 0.01 | <LOQ | 28.65 ± 0.05 | <LOQ | <LOQ | 9.85 ± 0.10 | <LOQ | <LOQ | 19.49 ± 0.02 | <LOQ | 16.13 ± 0.01 | 18.00 ± 0.02 | <LOQ | <LOQ | 18.28 ± 0.02 | <LOQ | <LOQ | <LOQ | <LOQ | 24.13 ± 0.04 | <LOQ |
| Kaempferol 3-[2′′′,3′′′,5′′′-triacetyl-alpha-L-arabinofuranosyl-(1->6)-glucoside isomer a | 105.09 ± 0.55 | 177.64 ± 0.69 | 853.59 ± 2.06 | 173.70 ± 0.69 | <LOQ | <LOQ | 44.77 ± 0.20 | 25.33 ± 0.15 | 12.54 ± 0.11 | 69.02 ± 0.48 | 31.35 ± 0.16 | 25.93 ± 0.04 | 172.97 ± 0.69 | 1.81 ± 0.08 | 885.25 ± 2.12 | 162.14 ± 0.66 | 608.30 ± 1.56 | 124.20 ± 0.59 | 68.78 ± 0.47 | <LOQ | 11.83 ± 0.11 | 23.04 ± 0.03 | 312.60 ± 0.97 |
| Kaempferol 3-[2′′′,3′′′,5′′′-triacetyl-alpha-L-arabinofuranosyl-(1->6)-glucoside isomer b | 466.33 ± 1.28 | 363.52 ± 1.07 | 1278.43 ± 2.92 | 372.00 ± 1.09 | <LOQ | <LOQ | 48.46 ± 0.43 | 4.13 ± 0.34 | 68.34 ± 0.27 | 298.90 ± 0.94 | 47.80 ± 0.43 | 30.47 ± 0.16 | 525.69 ± 1.40 | 24.02 ± 0.14 | 1390.73 ± 3.15 | 467.01 ± 1.28 | 1161.21 ± 2.68 | 360.48 ± 1.06 | 220.77 ± 0.78 | <LOQ | 61.33 ± 0.25 | 3.55 ± 0.09 | 699.38 ± 1.75 |
| Sum of phenolic acids | 2475.79 ± 5.32 | 2144.80 ± 4.45 | 3122.40 ± 6.34 | 3169.31 ± 5.91 | 138.05 ± 0.26 | 32.47 ± 0.06 | 1754.16 ± 3.90 | 1187.63 ± 3.30 | 1011.62 ± 2.93 | 2810.25 ± 5.07 | 1176.67 ± 3.31 | 1285.88 ± 3.26 | 2543.38 ± 5.33 | 837.81 ± 2.63 | 5561.52 ± 9.22 | 2418.55 ± 5.31 | 4195.52 ± 7.70 | 2615.70 ± 5.19 | 2104.76 ± 4.52 | 17.98 ± 0.04 | 1316.76 ± 3.38 | 637.14 ± 2.31 | 3063.90 ± 6.01 |
| Sum of flavonoids | 2046.21 ± 6.96 | 2630.38 ± 8.26 | 4190.25 ± 11.41 | 2175.23 ± 7.43 | 1217.99 ± 3.86 | 79.36 ± 0.56 | 1300.01 ± 4.56 | 1358.97 ± 5.74 | 675.77 ± 2.07 | 1713.71 ± 6.24 | 1180.55 ± 4.35 | 1273.63 ± 4.09 | 2288.59 ± 7.80 | 1442.58 ± 5.35 | 4200.24 ± 10.97 | 1819.87 ± 5.22 | 3447.67 ± 9.07 | 2173.75 ± 6.92 | 1896.80 ± 5.85 | 67.75 ± 0.24 | 1093.58 ± 3.90 | 1202.77 ± 4.17 | 2671.39 ± 7.98 |
| Sum of phenolic compounds | 4521.99 ± 12.29 | 4775.17 ± 12.71 | 7312.65 ± 17.75 | 5344.54 ± 13.34 | 1356.04 ± 4.12 | 111.82 ± 0.62 | 3054.18 ± 8.45 | 2546.60 ± 9.03 | 1687.39 ± 5.00 | 4523.96 ± 11.31 | 2357.22 ± 7.66 | 2559.51 ± 7.36 | 4831.97 ± 13.13 | 2280.39 ± 7.97 | 9761.76 ± 20.19 | 4238.42 ± 10.53 | 7643.19 ± 16.76 | 4789.45 ± 12.11 | 4001.56 ± 10.37 | 85.73 ± 0.29 | 2410.34 ± 7.28 | 1839.90 ± 6.48 | 5735.29 ± 13.99 |
LOD: limit of detection; LOQ: limit of quantification.