Figure 1.

Effect of IL-27, teriflunomide (TER), all-trans-retinoic acid (ATRA), and their combinations on the absolute number of CD4⁺ T cells under stimulated and unstimulated conditions. The parameter was determined in cell cultures incubated in the absence (CONTROL) and presence of IL-27 (100 ng/mL), TER (10⁻⁴ M), and ATRA (10⁻⁶ M) for 72 h including concomitant stimulation (with IL-2, anti-CD3, and anti-CD28 and re-stimulation with PMA and ionomycin for the last 5 h before harvest) (A) and for 96 h without stimulation (B). The absolute count represents the number of CD4⁺ T cells per sample well. Results are expressed as the mean (±S.D.) of three independent experiments with four mice per experiment (overall n = 12 (A) or n = 8 (B), * p < 0.05, ** p < 0.01, *** p < 0.001).