Figure 2.

Western blotting analysis of the expression level or activation status of proteins involved in the regulation of the cell cycle and in signal transduction pathways. (A) MSTO-211H cells were simultaneously treated with abemaciclib (0.5 µM), cisplatin (0.3 µM), and pemetrexed (0.02 µM) for 24 h. (B) H28 cells were treated with abemaciclib (1 µM), cisplatin (1 µM), and pemetrexed (0.05 µM) for 24 h. (C) ZS-LP cells were treated with abemaciclib (1 µM), cisplatin (1 µM), and pemetrexed (0.05 µM) for 24 h. At the end of the treatments, the expression levels of proteins involved in cell cycle regulation and signal transduction were analyzed by Western blotting. The immunoreactive spots were quantified by densitometric analysis; the results are represented by the ratio between phosphorylated and total protein levels or between the total protein and actin levels. The dash (-) represents a non-detectable signal. Data are representative of two independent experiments. Figure S5: Whole Western blots of Figure 2.