FIG. 1.
Western blotting of chinchilla sera and a murine monoclonal antibody versus NTHI strain 1128 whole OMP preparation (in all lanes). Chinchilla serum pools: lane 1, anti-whole OMP (delivered in CFA); lane 2, anti-isolated P5-fimbrin (delivered in CFA); lane 3, anti-isolated P5-fimbrin (delivered in A1PO4); lane 4, anti-LB1 (delivered in CFA); lane 5, anti-LPD-LB1(f)2,1,3 (delivered in A1PO4 plus MPL); lane 6, monoclonal antibody 2C7. Arrows indicate approximate recognition of the fully and partially denatured species of P5-fimbrin at 37 and 25 kDa, respectively. The major reactivity observed in lane 5 is directed against LPD.