Figure 8.

Sucrose density gradients showing the distribution of ENaC alpha subunit to lipid rafts after treating mpkCCD cells with uEVs from the active phase compared to the inactive phase. mpkCCD cells were challenged with uEVs from the inactive phase (A) or the active phase (B) before isolating lipid rafts and non-lipid rafts. Representative Western blots for ENaC alpha subunit protein expression in each fraction. Caveolin 1 (Cav1) is present in the light density gradient fractions corresponding to lipid rafts, while the mu-2 protein is present in non-lipid raft associated fractions. n = 2 independent experiments.