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. 2022 Nov 27;11(23):3794. doi: 10.3390/cells11233794

Figure 1.

Figure 1

IR dose-dependent phosphoproteome dynamics: (A) Statistics of phosphosites quantified in IB10 mouse ES cells. Phosphosites quantified and those that responded to DNA damage are depicted in the top and bottom bars, respectively. Light bar indicates total phosphosites and the dark bar indicates class I phosphosites (>0.75 phosphosite localization probability). (B) Principal component analysis (PCA) map of log2 SILAC ratios of total responsive phosphosites after LD (orange dots) and HD (blue dots) exposure over unirradiated controls. Four groups are readily visible. (C) Venn diagram of 9205 phosphosites that responded to LD (orange circle) and HD (blue circle). (D) Temporal dynamics showing the timing of phosphoproteome changes in LD- and HD-exposed mESCs. (E) Bar charts showing quantifications of ATM phosphosite pS1987 in response to LD and HD. Dots and bars represent quantifications and median values of up to six replicates, respectively, and error bars represent a 95% confidence interval (CI). (F) Density plot showing the distribution of the magnitude of global phosphoproteome changes in LD- and HD-exposed cells.