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Binding tests of HuscFv-NPs to (a) HUVECs and (b) KG-1a cells by flow cytometry. The percentage of RhB-positive cells of (c) HUVECs and (d) KG-1a cells, and mean fluorescence intensity (MFI) of conjugated-NPs binding to (e) HUVECs and (f) KG-1a cells. Positive and negative controls were anti-CD34 Ab-conjugated NPs (anti-CD34-NP) and RhB-loaded NPs (RhB NP), respectively. Data are presented as mean ± SD (n = 3). ** p < 0.01, *** p < 0.001 and **** p < 0.0001.
