Table 1.

Primer sequences of five species.

Bacteria	Primer Sequence (5′-3′)	Annealing Temperature	References
Total intestinal flora	F:ACTCCTACGGGAGGCAGCAG R:ATTACCGCGGCTGCTGG	64 °C	[21]
Escherichia coli	F:GTTAATACCTTTGCTCATTGA R:ACCAGGGTATCTTAATCCTGTT	60 °C	[22]
Lactobacillus	F:AGCAGTAGGGAATCTTCCA R:CACCGCTACACATGGAG	60 °C	[23]
Bifidobacterium	F:TCGCGTC(C/T)GGTGTGAAAG R:CCACATCCAGC(A/G)TCCAC	58 °C	[24]
Bacteroides	F:CTGAACCAGCCAAGTAGCG R:CCGCAAACTTTCACAACTGACTTA	68 °C	[25]