Figure 3.

Mebendazole promote apoptosis in pancreatic cancer cell through intrinsic mitochondrial pathway: (a) MIA Paca-2 cell line was treated with desired concentrations using MBZ, RB005, PF543 and FTY720 treatment for 24 h and apoptosis was analyzed by Annexin V-FITC; (b) JC-10 assay was performed to determine mitochondrial membrane potential (MMP) after treatment at a concentration of 20 µM; (c) analysis of apoptosis marker using western blotting and ImageJ software was used to quantify western blotting results and data were normalized with β-actin. Each experiment was repeated three times independently. Data were expressed on mean ± S.D, * p < 0.05, ** p < 0.01 and *** p < 0.001.