Figure 5.

Expression of marker proteins by hiPSC-derived Schwann cells for several stages of differentiation. (A–C) Cells were differentiated according to the protocol presented by Hörner et al., 2021 [118], which is depicted in Figure 4. Representative immunofluorescence images of cells stained for different marker proteins (indicated) during the differentiation phase (d12; d25) and the maturation phase (>d50). All microscopy images were obtained from our own work; parts of the figure were adapted with modifications from Hörner et al., 2021 [118] under CC BY 4.0 license. (A) Representative marker protein signals at the neural crest stage (d12) and Schwann cell precursor stage (d25). Timepoints of fixation and color codes for immunofluorescence signals are indicated at the top of panels. Vim = vimentin. Scale bars, 100 µm. (B) Representative marker protein signals of cells differentiated for more than 50 days in the expansion phase (left two panels) and the maturation phase (right two panels) of the protocol. Color codes for immunofluorescence signals are indicated at the top of panels. Scale bars, 100 µm. (C) Immunofluorescence images of single marker proteins, as indicated at the top of panels, to show marker distribution in differentiated Schwann cells after the maturation phase. Immunofluorescence signals are shown in greyscale. Cell nuclei were labeled with DAPI and are shown in red. Scale bars, 100 µm.