Table 1.
Studies describing primary protocols for Schwann cell differentiation from hiPSC and/or hESC. Sorted from the most to least recent.
| Study | Cell Source (Number of Cell Lines) |
Duration | Characterization | Schwann Cell Markers | Sorting Steps/Yield |
|---|---|---|---|---|---|
| Mukherjee-Clavin et al., 2019 [119] | hESC (1 control, 2 disease) hiPSC (2 control, 3 disease) |
approx. 55 d (21 d to SCP, up to +80 d in culture; SC maturation after 55 d in total) |
IF, RT-qPCR, microarray, RNA-seq, in vitro myelination assay, in vivo transplantation assay | PMP22, SOX10, MPZ, S100b, GFAP, GalC, MBP | 13.5% CD49d+ SCP at d21–23 (for hiPSC), are FACS purified for further culture; final SC yield not reported |
| Kim et al., 2017 [121] | hESC (3) hiPSC (1) |
31 d to SC identity | IF, RT-qPCR, microarray, neurotrophic factors secretion (ELISA), in vitro myelination assay, in vivo transplantation assay | GAP43, SOX10, p75NTR, MPZ, S100b, PMP22, KROX20, GFAP | no sorting, 99% SOX10+ (SCP stage), final SC yield not reported |
| Liu et al., 2012 [111] Liu et al., 2014 [235] |
hESC (2) hiPSC (1) |
>85 d (14 d to NCC, at least 30 d expansion, 40 d to SC) |
IF, RT-PCR/qPCR, microarray, in vitro myelination assay | GFAP, S100b, p75NTR, PMP22, MBP | FACS for p75NTR+ to purify NCC at d14, 85% S100b+ at d75 |
| Ziegler et al., 2011 [112] | hESC (1) | 98 d | IF, RT-PCR, neuron coculture (alignment with neurites) | GFAP, S100, p75NTR, Krox20, PMP22, MPZ, MBP | no sorting, 60% GFAP+/S100+ |
Abbreviations: d = days of differentiation, SCP = Schwann cell precursors, SC = Schwann cells, NCC = neural crest cells, IF = immunofluorescence.