Table 2.
Gene annotation and KEGG pathway of the differentially expressed genes in S. aureus ATCC6538 in response to CFS from L. rhamnosus SCB0119.
| Gene ID | RPKM Control | RPKM CFS | log2 Ratio | Gene Annotation |
|---|---|---|---|---|
| Fatty acid degradation | ||||
| EKM74_RS11915 | 1832.07 ± 490.15 | 697.14 ± 121.09 | −1.39396 | adhP; alcohol dehydrogenase |
| DNA replication | ||||
| EKM74_RS04795 | 85.2 ± 44.94 | 39.85 ± 1.91 | −1.09611 | single-stranded DNA-binding protein |
| Ribosome | ||||
| EKM74_RS01000 | 77.06 ± 19.54 | 474.46 ± 30.77 | 2.622215 | accC; acetyl-CoA carboxylase biotin carboxylase subunit |
| EKM74_RS01125 | 36.7 ± 12.69 | 642.43 ± 184.29 | 4.129847 | rpmG; 50S ribosomal protein L33 |
| EKM74_RS01310 | 21.83 ± 0.96 | 168.95 ± 53.18 | 2.951911 | rpsT; 30S ribosomal protein S20 |
| EKM74_RS01660 | 458.35 ± 97.45 | 2756.92 ± 263.1 | 2.588527 | rpmA; 50S ribosomal protein L27 |
| EKM74_RS01670 | 102.05 ± 29.64 | 4057 ± 488.97 | 5.313122 | rplU; 50S ribosomal protein L21 |
| EKM74_RS01855 | 19.42 ± 10.05 | 621.08 ± 94.23 | 4.999194 | rplT; 50S ribosomal protein L20 |
| EKM74_RS01860 | 51.94 ± 21.24 | 5478.49 ± 45.03 | 6.720704 | rpmI; 50S ribosomal protein L35 |
| EKM74_RS02080 | 80.66 ± 17.27 | 3451.94 ± 642.32 | 5.419314 | rpsD; 30S ribosomal protein S4 |
| EKM74_RS05465 | 190.13 ± 11.18 | 3317.01 ± 500.37 | 4.124828 | rpsI; 30S ribosomal protein S9 |
| EKM74_RS05470 | 150.17 ± 31.96 | 5880.76 ± 17.12 | 5.291357 | rplM; 50S ribosomal protein L13 |
| EKM74_RS05495 | 122.74 ± 4.32 | 3341.82 ± 309.69 | 4.766903 | rplQ; 50S ribosomal protein L17 |
| EKM74_RS05505 | 287.56 ± 78.6 | 2321.16 ± 704.2 | 3.01292 | rpsK; 30S ribosomal protein S11 |
| EKM74_RS05510 | 582.25 ± 5.82 | 10,572.69 ± 1079.55 | 4.182553 | rpsM; 30S ribosomal protein S13 |
| EKM74_RS05515 | 259.55 ± 26.92 | 8724.69 ± 798.78 | 5.071017 | rpmJ; 50S ribosomal protein L36 |
| EKM74_RS05535 | 328.44 ± 51.85 | 2770.35 ± 239.31 | 3.076378 | rplO; 50S ribosomal protein L15 |
| EKM74_RS05540 | 370.08 ± 105.31 | 13,564.79 ± 1089.74 | 5.195877 | rpmD; 50S ribosomal protein L30 |
| EKM74_RS05545 | 183.9 ± 22.05 | 4254.61 ± 754.45 | 4.532035 | rpsE; 30S ribosomal protein S5 |
| EKM74_RS05550 | 205.01 ± 26.43 | 9035.82 ± 328.78 | 5.461917 | rplR; 50S ribosomal protein L18 |
| EKM74_RS05555 | 81.16 ± 4.22 | 1809.37 ± 428.25 | 4.478643 | rplF; 50S ribosomal protein L6 |
| EKM74_RS05560 | 98.44 ± 3.27 | 3014.88 ± 247.03 | 4.93666 | rpsH; 30S ribosomal protein S8 |
| EKM74_RS05570 | 126.29 ± 2.95 | 4279.22 ± 391.93 | 5.082565 | rplE; 50S ribosomal protein L5 |
| EKM74_RS05575 | 138.6 ± 11.64 | 4600.35 ± 70.63 | 5.052719 | rplX; 50S ribosomal protein L24 |
| EKM74_RS05580 | 181.27 ± 11.93 | 4136.6 ± 837.23 | 4.512211 | rplN; 50S ribosomal protein L14 |
| EKM74_RS05585 | 140.46 ± 16.11 | 4981.9 ± 113.47 | 5.148408 | rpsQ; 30S ribosomal protein S17 |
| EKM74_RS05590 | 154.79 ± 34.38 | 4723.29 ± 418.72 | 4.931395 | rpmC; 50S ribosomal protein L29 |
| EKM74_RS05595 | 194.99 ± 8.39 | 3442.61 ± 618.09 | 4.142009 | rplP; 50S ribosomal protein L16 |
| EKM74_RS05600 | 333.68 ± 100.38 | 6017.46 ± 493.68 | 4.172617 | rpsC; 30S ribosomal protein S3 |
| EKM74_RS05605 | 267.84 ± 59.12 | 9325.05 ± 0.95 | 5.121682 | rplV; 50S ribosomal protein L22 |
| EKM74_RS05610 | 210.47 ± 4.59 | 7349.55 ± 191.55 | 5.125963 | rpsS; 30S ribosomal protein S19 |
| EKM74_RS05615 | 231.04 ± 32.27 | 4313.16 ± 576.59 | 4.222508 | rplB; 50S ribosomal protein L2 |
| EKM74_RS05620 | 87.68 ± 7.24 | 1918.56 ± 543.34 | 4.451662 | rplW; 50S ribosomal protein L23 |
| EKM74_RS05625 | 145.16 ± 12.67 | 3427.73 ± 563.02 | 4.561522 | rplD; 50S ribosomal protein L4 |
| EKM74_RS05630 | 188.98 ± 15.87 | 7819.97 ± 221.75 | 5.37082 | rplC; 50S ribosomal protein L3 |
| EKM74_RS05635 | 294.61 ± 86.56 | 9259.91 ± 978.26 | 4.97412 | rpsJ; 30S ribosomal protein S10 |
| EKM74_RS07510 | 151.39 ± 9.3 | 705.28 ± 120.89 | 2.219912 | rplI; 50S ribosomal protein L9 |
| EKM74_RS07590 | 114.58 ± 17.68 | 1144.54 ± 138.16 | 3.320336 | rpmH; 50S ribosomal protein L34 |
| EKM74_RS10450 | 236.72 ± 37.3 | 7542.84 ± 237.11 | 4.993827 | rpsF; 30S ribosomal protein S6 |
| EKM74_RS10460 | 55.84 ± 4.69 | 531.48 ± 99.45 | 3.250636 | rpsR; 30S ribosomal protein S18 |
| EKM74_RS11530 | 51.86 ± 16.74 | 493.86 ± 62.28 | 3.251456 | rpmG; 50S ribosomal protein L33 |
| EKM74_RS11545 | 230.05 ± 33.59 | 6745.81 ± 496 | 4.873994 | rplK; 50S ribosomal protein L11 |
| EKM74_RS11550 | 89.54 ± 9.77 | 1345.42 ± 304.26 | 3.909295 | rplA; 50S ribosomal protein L1 |
| EKM74_RS11560 | 75.58 ± 8 | 6432.15 ± 34.02 | 6.411134 | rplJ; 50S ribosomal protein L10 |
| EKM74_RS11565 | 38.15 ± 13.06 | 2351.81 ± 364.44 | 5.945945 | rplL; 50S ribosomal protein L7/L12 |
| EKM74_RS11590 | 185.09 ± 16.78 | 6759.64 ± 1120.81 | 5.190643 | rpsL; 30S ribosomal protein S12 |
| EKM74_RS11595 | 156.22 ± 21.95 | 3889.22 ± 770.69 | 4.637864 | rpsG; 30S ribosomal protein S7 |
| EKM74_RS14380 | 74.47 ± 6.98 | 956.98 ± 89.75 | 3.683755 | rpmF; 50S ribosomal protein L32 |
| EKM74_RS15340 | 44.89 ± 6.07 | 387.7 ± 59.81 | 3.110434 | rpsP; 30S ribosomal protein S16 |
| EKM74_RS15355 | 56.77 ± 3.55 | 839.03 ± 212.16 | 3.885462 | rplS; 50S ribosomal protein L19 |
| EKM74_RS15435 | 112.72 ± 30.02 | 3718.42 ± 314.52 | 5.043867 | rpsB; 30S ribosomal protein S2 |
| EKM74_RS15530 | 178.88 ± 2.05 | 2219.27 ± 90.62 | 3.633007 | rpsO; 30S ribosomal protein S15 |
| Aminoacyl-tRNA biosynthesis | ||||
| EKM74_RS02305 | 35.37 ± 21.65 | 401.03 ± 43.46 | 3.503254 | leuS; leucine-tRNA ligase |
| EKM74_RS03605 | 63.4 ± 16.82 | 997.64 ± 109.83 | 3.975903 | gatB; Asp-tRNA (Asn)/Glu-tRNA (Gln) amidotransferase subunit |
| EKM74_RS11370 | 90.84 ± 8.58 | 509.92 ± 53.53 | 2.488778 | lysS; lysine-tRNA ligase |
| EKM74_RS11490 | 107.77 ± 37.9 | 605.02 ± 32.1 | 2.489052 | gltX; glutamate-tRNA ligase |
| EKM74_RS14775 | 34.9 ± 14.25 | 239.53 ± 20.28 | 2.77887 | pheT; phenylalanine-tRNA ligase subunit beta |
| EKM74_RS15105 | 30.17 ± 17.78 | 273.57 ± 29.78 | 3.180715 | ileS; isoleucine-tRNA ligase |
| Oxidative phosphorylation | ||||
| EKM74_RS04830 | 170.47 ± 3.15 | 3033.08 ± 240.81 | 4.153207183 | atpD; F0-F1 ATP synthase subunit beta |
| EKM74_RS04840 | 97.18 ± 1.86 | 1251.21 ± 170.89 | 3.686521282 | atpA; F0-F1 ATP synthase subunit alpha |
| EKM74_RS04855 | 241.14 ± 56.65 | 1679.25 ± 147.57 | 2.799862175 | atpE; F0-F1 ATP synthase subunit C |
| EKM74_RS04860 | 239.15 ± 52.69 | 1550.63 ± 98.56 | 2.696892312 | atpB; F0-F1 ATP synthase subunit A |