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. 2022 Nov 29;11(23):3854. doi: 10.3390/foods11233854

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Circular (A) and linear (B) sequence alignment of mcr-1-bearing IncHI2 plasmids in this study and other similar plasmids available from the NCBI database. (A) Circular alignment of the nine pMCR_S46L1-like plasmids, pMCR_S58L2 and similar plasmids available from the NCBI database including pHNSHP45-2 (KU341381), pSH16G1394 (NZ_MK477614) and plas4.1.1 (NZ_CP047116). Plasmid pHNSHP45-2 was included as a reference and the outermost ring showed annotations of pHNSHP45-2. The resistance genes and transfer-related genes are marked with red and purple, respectively. (B) Linear alignment of mcr-1-harboring pMCR_S46L1 (CP091541), pMCR_S58L2 (CP091543) and plas4.1.1 (NZ_CP047116). Insertion sequences or tnpA in insertion sequences are highlighted in orange arrows, while resistance genes are indicated by red arrows.