TABLE 6.
Serum neutralizing activity against in vitro cytotoxicity of CDTA for HT-29 cellsa
| Immunogen | μg of Ab/ml (ELISA) | Reciprocal neutralization titer
|
|
|---|---|---|---|
| GM | Range | ||
| Pn14-rARU | 194 | 104 | 64–256 |
| Pn14-rARUsucc | 371 | 111 | 64–128 |
| SF-rARU | 613 | 194 | 64–256 |
| SF-rARUsucc | 518 | 111 | 64–256 |
| K1-rARUsucc | 390 | 181 | 64–256 |
| Goat antitoxin (0.5 mg/ml)b | 128 | ||
| PBS | 0 | ||
Neutralizing titers were the highest serum dilution that completely inhibited the cytotoxicity of CDTA (20 ng/well) on HT-29 cells (Materials and Methods). The titers represent the geometric means (GM) ± standard deviations from general-purpose Swiss Albino mice (n = 10) obtained 7 days after the third injection. Anti-CDTA was also measured by ELISA, and the individual values are expressed as micrograms of Ab per milliliter of serum.
Affinity-purified goat Ab.