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. 2000 Apr;68(4):2161–2166. doi: 10.1128/iai.68.4.2161-2166.2000

TABLE 6.

Serum neutralizing activity against in vitro cytotoxicity of CDTA for HT-29 cellsa

Immunogen μg of Ab/ml (ELISA) Reciprocal neutralization titer
GM Range
Pn14-rARU 194 104 64–256
Pn14-rARUsucc 371 111 64–128
SF-rARU 613 194 64–256
SF-rARUsucc 518 111 64–256
K1-rARUsucc 390 181 64–256
Goat antitoxin (0.5 mg/ml)b 128
PBS 0
a

Neutralizing titers were the highest serum dilution that completely inhibited the cytotoxicity of CDTA (20 ng/well) on HT-29 cells (Materials and Methods). The titers represent the geometric means (GM) ± standard deviations from general-purpose Swiss Albino mice (n = 10) obtained 7 days after the third injection. Anti-CDTA was also measured by ELISA, and the individual values are expressed as micrograms of Ab per milliliter of serum. 

b

Affinity-purified goat Ab.