Table 4.
Microsatellite loci, primer sequences designed from the flanking region of the SSR sequences identified, and annealing temperatures (Tm).
| Locus | Product Size (bp) | Type of Repeat | Length | Tm | Orientation | Primer Sequence (5′-3′) |
|---|---|---|---|---|---|---|
| RPRSCP1 | 167 | (A)13 | 20 | 55.01 | Forward | ATCGGAAGATCCTCTTTTTC |
| 20 | 54.95 | Reverse | AGCTGTATTGTATGCGGAAT | |||
| RPRSCP2 | 176 | (TA)8 | 20 | 54.15 | Forward | TAAGGTGGTAACTCCCATTC |
| 20 | 54.73 | Reverse | AACAAGAGGATTGGTTCTCA | |||
| RPRSCP3 | 241 | (TA)5 | 20 | 54.90 | Forward | GTTAATGAAAGAGCCCAATG |
| 20 | 54.62 | Reverse | CCATCGATCTTGATAAGGAC | |||
| RPRSCP4 | 229 | (T)13 | 20 | 55.12 | Forward | GAAGTATCTGTCCGATCCAA |
| 20 | 54.35 | Reverse | GTTCCGAACTAGACGATGTT | |||
| RPRSCP5 | 250 | (TA)5 | 20 | 56.03 | Forward | ACAGAATCGTGGTGAATCAG |
| 20 | 54.91 | Reverse | GGATAGCGAGTATTGTCCAG | |||
| RPRSCP6 | 194 | (AT)7 | 20 | 54.94 | Forward | GTCTCTCTTCAGAGCGAAAA |
| 20 | 55.00 | Reverse | GTACCCCGTGATCTCAATAA | |||
| RPRSCP7 | 163 | (AT)5 | 20 | 55.02 | Forward | GTAAACCAAGAAGCCCCTAT |
| 20 | 55.02 | Reverse | CTTCTTCCATTTCTCGATTG | |||
| RPRSCP8 | 202 | (CT)7 | 20 | 54.97 | Forward | CAGGAAAAAGAGCTGAAGAA |
| 20 | 55.05 | Reverse | AGGGTAGATCGGGATAATGT | |||
| RPRSCP9 | 231 | (A)11 | 20 | 55.03 | Forward | CCAATCCAATGTGAGAAAGT |
| 20 | 54.95 | Reverse | CATTGGATCAAGAACAGGAT | |||
| RPRSCP10 | 207 | (T)15 | 20 | 54.44 | Forward | TTTCCTTAGTTTCCATCGAC |
| 20 | 54.40 | Reverse | CGAGAAAGGTGTTTGGTAAT | |||
| RPRSCP11 | 236 | (T)14 | 20 | 55.06 | Forward | CATTGCAGGTACAATGACAG |
| 20 | 54.89 | Reverse | TCGGAAGAGGAATAGGTACA | |||
| RPRSCP12 | 245 | (T)14 | 20 | 55.07 | Forward | CAGAGGTCAATTTCTTCTGC |
| 20 | 54.82 | Reverse | GAAAAAGGAGGAAAGAGAGG | |||
| RPRSCP13 | 213 | (T)16 | 20 | 54.79 | Forward | GATGGCTAGAGATTCATTGG |
| 20 | 55.23 | Reverse | ATTGAGCTGACATCCGTTAC | |||
| RPRSCP14 | 234 | (T)12 | 20 | 54.96 | Forward | AACAGGTATGGTTGGTATCG |
| 20 | 55.21 | Reverse | AGCCGAGCTATTCTCTTTTT | |||
| RPRSCP15 | 360 | (C)10 | 20 | 55.03 | Forward | TATCTGATCCTCGAATCACC |
| 20 | 55.11 | Reverse | ATCGGACCACGATGTAGTAG | |||
| RPRSCP16 | 214 | (T)13 | 20 | 54.57 | Forward | GTGATCCAAAAGTGAAAACC |
| 20 | 55.43 | Reverse | CGAATTACGGACAACCTAAA | |||
| RPRSCP17 | 229 | (AGGT)3 | 19 | 54.89 | Forward | TGAAGTAACCCATGCCATA |
| 20 | 55.12 | Reverse | GGAGACCTGTGTTTTTGGTA | |||
| RPRSCP18 | 234 | (TAT)4 | 20 | 55.03 | Forward | ACACCCCACCCTAGAGTTAT |
| 20 | 55.33 | Reverse | GGGCGACTGAGATATTACAA | |||
| RPRSCP19 | 249 | (AT)4 | 22 | 50.33 | Forward | CTCCTAGATAAGCTAACAGAGA |
| 20 | 56.33 | Reverse | TCGAAACTCCTTGTTGATTG | |||
| RPRSCP20 | 360 | (ATGAA)3 | 20 | 55.85 | Forward | ACATCGGTGACAAAGATGAC |
| 20 | 55.16 | Reverse | GTTCTTCTTTCGGAAGTCCT | |||
| RPRSCP21 | 221 | (T)13 | 20 | 55.78 | Forward | CGCAGTATGGGTCTAGCTTA |
| 20 | 54.92 | Reverse | GCAGATATGGGCAAACTAAC | |||
| RPRSCP22 | 229 | (AT)10 | 20 | 53.61 | Forward | TCCTTTTCCGTATACTTTCC |
| 20 | 54.93 | Reverse | CGGGTTAATGTGAGCTTATC | |||
| RPRSCP23 | 239 | (AAAG)3 | 20 | 55.12 | Forward | AGGTTCGAGTCAAATAGCAA |
| 20 | 55.37 | Reverse | AACCGTACATACGACTTTCG | |||
| RPRSCP24 | 229 | (T1)12 | 20 | 55.17 | Forward | GGACATGTGGAAAAGAGAAA |
| 20 | 55.37 | Reverse | GCGCATGTATAAGACCAAAT | |||
| RPRSCP25 | 177 | (AT)8 | 21 | 55.12 | Forward | CGATATCAATACTCGAAGACG |
| 20 | 54.75 | Reverse | TGTCTACCATTTCACCATCA | |||
| RPRSCP26 | 210 | (T)12 | 20 | 55.87 | Forward | GATCTCGGAGTGAAGAACCT |
| 20 | 54.81 | Reverse | GAAAGAGCAATGGAATATGG |