Figure 3.

Microglial migration competency by cell scratch assay. The BV2 cells with the genotypes of IRF1-WT, IRF1-KO (BV2^ΔIRF1), and IRF1-OE (overexpression) were cultivated to confluency. The cells were physically removed by scratching, and then the cell images were taken by differential interference contrast (DIC) microscopy at 24 and 48 h (A). 40× magnification. The cells in the scratched areas were quantified (B). N = 3; * p < 0.05; *** p < 0.001 (IRF1-WT vs. IRF1-KO). $ p < 0.05 (IRF1-OE vs. IRF1-WT).