Figure 4.
A. vulgaris extract displays cytocidal effects as well as inhibition of proliferation of the A549 cell line. Cells were exposed to an IC50 dose of A. vulgaris extract for 72 h, and flow cytometric analyses of (A) Ann/PI staining, (B) DAPI staining on chamber slides, (C) Apostat staining, (D) CFSE staining, and (E) AO staining were performed. White arrows mark apoptotic nuclei. (F) Cell viability determination after treatment with A. vulgaris extract alone and in combination with autophagy inhibitor 3-MA by MTT assay. * p < 0.05 was considered statistically significant in comparison to the control.