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. 2022 Dec 3;23(23):15258. doi: 10.3390/ijms232315258

Figure 4.

Figure 4

Microarray system designed to test simultaneously (multiplex) all three CC oligonucleotide pairs assayed in both formats (PPRH and duplex clamp). (A): Scheme of the chip organization and the concentrations used to build standard curves for each CC pair are shown together with a fluorescence image of a microarray well. (B): Calibration curve obtained for the CC1 pair and the corresponding analytical parameters of this sigmoidal curve obtained in a multiplex microarray assay (RFU: relative fluorescence units) are also plotted. All standard curves obtained were designed to record at least 3 spot replicates for each concentration value.