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. 2022 Dec 3;23(23):15258. doi: 10.3390/ijms232315258

Figure 9.

Figure 9

Testing of six real samples provided by the Biobank of the Aragon Health System (3 negatives and 3 positives by PCR: sample #1, Ct 35; sample #2, Ct 19 and sample #3, Ct 30) in UTM from Biocomma with the PPRH-CC1 as capture sequence using the two developed biosensor devices. (A): Profile of ipeak® (IUL S.A. commercial lateral flow reader) quantification (grey intensity level measurements, right side) of the results obtained with the TLF together with the signal obtained for the blank and control of 0.001 nM of target DNA CC1 sequence and the image obtained from the back part of the LF strips (thermosensitive material, left side) after the laser irradiation using a 1064 nm laser used. This image was used to measure the grey level intensity to develop the results of the thermal lateral flow assay. (B) Analytical signal values obtained by the electrochemical biosensor platform, together with the signal obtained for the blank and control of 0.41 nM of target DNA CC1 sequence. Standard deviation of three replicates carried out consecutively is drawn as error bars.