Table 1.
Adult C. lividipennis female hemolymph’s free amino acids contents under different injection treatments of the same volume of PBS, dsGFP, and dsPSP for 2 days.
| Amino Acids | Concentration (μmol/mL) | ||
|---|---|---|---|
| PBS | dsGFP | dsPSP | |
| Asparagine (Asp) | 15.05 ± 1.52 a | 15.77 ± 1.66 a | 14.32 ± 1.09 a |
| Glutamicacid (Glu) | 18.41 ± 1.36 a | 18.62 ± 1.69 a | 17.81 ± 1.75 b |
| Serine (Ser) * | 14.65 ± 1.42 a | 14.47 ± 0.94 a | 8.14 ± 0.95 b |
| Arginine (Arg) * | 18.87 ± 1.65 a | 18.47 ± 1.46 a | 13.46 ± 1.26 b |
| Glycine (Gly) | 10.09 ± 0.78 a | 9.86 ± 0.74 a | 9.76 ± 0.79 a |
| Proline (Pro) | 11.60 ± 1.02 a | 10.14 ± 0.95 a | 12.71 ± 0.93 a |
| Alanine (Ala) | 20.98 ± 1.89 a | 21.74 ± 2.05 a | 19.39 ± 1.84 a |
| Threonine (Thr) * | 8.68 ± 0.97 a | 8.72 ± 1.02 a | 11.23 ± 0.92 b |
| Valine (Val) | 0.46 ± 0.036 b | 0.43 ± 0.024 b | 1.57 ± 0.13 a |
| Methionine (Met) | 11.34 ± 1.12 a | 11.98 ± 1.07 a | 9.85 ± 0.92 a |
| Cystine (Cys) * | 56.53 ± 3.12 b | 56.67 ± 3.56 b | 71.27 ± 4.39 a |
| Isoleucine (Ile) | 10.41 ± 1.16 a | 10.76 ± 0.96 a | 9.73 ± 0.82 a |
| Leucine (Leu) | 15.05 ± 1.29 b | 16.00 ± 1.03 b | 16.24 ± 1.34 a |
| Phenylalanine (Phe) | 10.83 ± 0.95 a | 10.51 ± 1.12 a | 9.48 ± 0.87 a |
| Histidine (His) | 5.83 ± 0.36 a | 4.73 ± 0.23 a | 4.94 ± 0.47 a |
| Lysine (Lys) | 10.16 ± 0.93 a | 10.03 ± 0.85 a | 8.82 ± 0.85 a |
| Tyrosine (Tyr) * | 8.68 ± 0.78 b | 8.39 ± 0.88 b | 12.00 ± 1.09 a |
| Total | 246.4 ± 13.43 a | 247.8 ± 11.57 a | 249.5 ± 12.53 a |
Abbreviations: GFP, green fluorescent protein; PBS, phosphate buffer saline (10 μmol/mL). * Shows significant difference. Note: The amount of free amino acids in the hemolymph was measured using an Amino Acid Analyzer (Beckman 6300). ANOVA and the Tukey–Kramer test were used to evaluate the variation in each amino acid quantity between treatments. The data that do not share the same lowercase letters are significantly different at p < 0.05 (Tukey t-test).