Skip to main content
. 2022 Dec 2;27(23):8475. doi: 10.3390/molecules27238475

Figure 1.

Figure 1

Animal models of RA-ILD were constructed, and the expression of autophagy-related proteins was changed. (A) CFA and chicken collagen type II were subcutaneously injected into the tail root of C57BL/6 mice on day 0 and 21 and then treated with bleomycin on day 25. On day 35, the mice were sacrificed. (B) The lung weight was measured. (C,D) Representative H&E and Masson’s staining images of lungs from the three groups. Scale bars = 50 μm, shown at 100× (top panels) and 200× (bottom panels). (E) The levels of hydroxyproline in the lung were measured. (F) The mRNA levels of IL-1β, Col1A1, Col3A1, FN-1, and TGF-β were quantified. (G) The levels of HIF-1a, BECN1, BNIP3L, BNIP3, LC3 I, and LC3 II in the lungs were detected using immunoblotting. The ratios of LC3 II/I were quantified as described above. Values represent the mean ± SEM (n = 3–5). Compared with the Ctrl group, * p < 0.05, ** p < 0.01, and *** p < 0.001; compared with the model group (BLM), # p < 0.05 and ## p < 0.01.