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. 2022 Dec 6;23(23):15402. doi: 10.3390/ijms232315402

Figure 3.

Figure 3

Ino80 depletion in differentiating ESCs causes extension of S-phase and apoptosis. (A) Schematic images showing differentiation protocol of differentiating ESCs, and representative images showing expansion of differentiating cells from for EBs. Images were captured at day 5, 7, 9, 10, and 12 after seeding same numbers of EBs on gelatin-coated dishes. Scale bar, 200 μm. EBs, embryoid bodies. (B) Representative images of apoptosis analysis in Diff−CONT and Diff−iKO ESCs and quantification (N = 3). The difference in apoptosis rates between Diff−CONT and Diff−iKO ESCs is 19.57% on average. Diff−CONT ESCs, differentiating ESCs from CONT ESCs. Diff−iKO ESCs, differentiating ESCs from Ino80 iKO ESCs. (C) Representative cell cycle histogram of Diff−CONT and Diff−iKO ESCs and their percentages of G1, S, and G2/M phases (N = 5). Statistical significance was determined at a p-value lower than 0.05 (*) or 0.0001 (****).