TABLE 2.

PCR primers and templates for probe preparation and genomic sequence analysis

PCR product	Size (bp)	PCR primera		PCR template
		Left	Right
eibA probe	1,000	pr172	pr173	pCS6379
eibE probe	785	pr219	pr234	CH6249
eaaA probe	737	Standard vector	Standard vector	pDC6181
eaaC min 42.0b	1,250	pr196	pr197	CH6304
K-12 min 42.0b	989	pr221	pr197	K-12

^aPrimer sequences were as follows: pr172, CTGTTTTGTCTGCGGCAATGGC; pr173, TTACCCACGCTGTACGGCTGGAAC; pr196, GGCCGTATGCTGATGTCTGTG; pr197, CCTGGGAACTTGGCTTTAATC; pr219, CTAGGACGGCAAAGTGTG; pr221, CGATGGCACCTGTCTATG; pr234, ACGCCCATGTTGTAGGAC. 

^bThe primers for amplifying the insert end and chromosomal attachment site relative to eibC were based on the sequences of eaaC (pr196) and the bacterial yecN (pr221) and yecD (pr197) genes.