Table 1.
The primer sequences used for QPCR analysis.
| For Gallus gallus | |||
| Gene name | Forward | Backward | Accession No. |
| CD44 | CCGGGCTTTTCTTCCTTCTG | AGTTGGCCATTGTTTCCTCAG | NM_204860.5 |
| CD73 (NT5E) | CTCCCGTTTCAAGGGTCAGG | TTCATGGTTGCCCAAAGCCA | XM_040669143.1 |
| CD71 (TFRC) | TTGGAGACTCCTGATGCTATCG | TCACATAGACAGGTTTGCCAGA | NM_205256.2 |
| CD34 | TGGGAGTAAGAGTGGGGTCG | CCTGGAGCAGAAAAGGGACTT | XM_040691711.1 |
| MKI67 (Ki-67) | AAACGGAATGGGACTGACGG | TCACATTCTGTTCTCCTTCCAAA | XM_040674390.2 |
| PCNA | CGGATACGTTGGCTCTAGTGT | GGAATTCCAAGCTGCTCCAC | NM_204170.3 |
| RAB27A | AGAAAAAGGCAAATGTGGCTGC | GAGAGTTCACTAAGGCTGCATGA | FJ449551.1 |
| GAPDH | GTCAAGGCTGAGAACGGGAA | GCCCATTTGATGTTGCTGGG | NM_204305.2 |
| For Homo sapiens | |||
| Gene name | Forward | Backward | Accession No. |
| COL1A1 | GTCAGATGGGCCCCCG | CACCATCATTTCCACGAGCA | NM_000088.4 |
| COL3A1 | GAGGATGGTTGCACGAAACAC | CAGCCTTGCGTGTTCGATATT | NM_000090 |
| ELN | CAGGTGCGGTGGTTCCTC | CTGGGTATACACCTGGCAGC | M36860 |
| IL-1β | GCAGCCATGGCAGAAGTACC | AGTCATCCTCATTGCCACTGTAAT | NM_000576.2 |
| TNF-α | TAGCCCATGTTGTAGCAAACCC | TTATCTCTCAGCTCCACGCCA | NM_000594.3 |
| IL-6 | ACCCCCAGGAGAAGATTCCA | GATGCCGTCGAGGATGTACC | M54894.1 |
| RAC1 | TGGCTAAGGAGATTGGTGCTG | CGGATCGCTTCGTCAAACAC | AF498964.1 |
| IGF-1 | ATCAGCAGTCTTCCAACCCAAT | GCCAGGTAGAAGAGATGCGA | M29644.1 |
| CCND1 | ATCAAGTGTGACCCGGACTG | CTTGGGGTCCATGTTCTGCT | NM_053056.3 |
| PCNA | TCTGAGGGCTTCGACACCTA | TCATTGCCGGCGCATTTTAG | BC062439.1 |
| CASP2 | GCATGTACTCCCACCGTTGA | GACAGGCGGAGCTTCTTGTA | NM_032982.3 |
| TP53 | AAGTCTAGAGCCACCGTCCA | CAGTCTGGCTGCCAATCCA | NM_000546.5 |
| 18s | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG | NR_003286 |
Abbreviation: NT5E: 5′-nucleotidase ecto; TFRC: transferrin receptor; PCNA: proliferating cell nuclear antigen; GAPDH: glyceraldehyde-3-phosphate dehydrogenase. COL1A1: Human Collagen type I α1; COL3A1: Human Collagen type III α1; ELN: Human Elastin; IL-1β: interleukin 1 β; TNF-α: tumor necrosis factor α; IL-6: interleukin 6; IGF-1: insulin-like growth factor I; CCND1: cyclin D1; PCNA: proliferating cell nuclear antigen; CASP2: TP53: tumor protein p53. Symbols for genes are italicized.