FIG. 2.

Variability in sporozoite viability and/or infectivity between sporozoites isolated from different batches of infected mosquitoes. Groups of mice were challenged with 5.0 × 10⁴ sporozoites, and liver parasite burden was measured by Taq Man quantification of parasite-specific 18S rDNA at 42 h postinfection (A). Each experiment represents a group of mice challenged with sporozoites isolated from a different batch of infected mosquitoes. Equal cDNA synthesis between groups of mice was ensured by quantification of the housekeeping gene GAPDH (B). Experiment 1 represents data from immunocompetent C57BL/6 mice challenged with 5.0 × 10⁴ sporozoites also displayed in Fig. 1. Experiment 3 represents data from immunocompetent C57BL/6 mice challenged with 5.0 × 10⁴ sporozoites also displayed in experiment 1 of Table 1. Experiment 4 represents data from immunocompetent C57BL/6 mice challenged with 5.0 × 10⁴ sporozoites also displayed in Fig. 4 and experiment 2 of Table 1.