Figure 2.

PAP-1 attenuated epileptic seizures and hippocampal neuronal damage in KA-induced mouse epilepsy model. (A) Flowchart of the experiment in vivo. (B) Seizure severity of mice after KA treatment in the presence (n = 9) or absence of PAP-1 (n = 10); statistics by Mann–Whitney test. (C) Seizure latency to SE; statistics by unpaired t-test. (D,F,H) Representative HE, Nissl, and NeuN staining of the hippocampal CA3 region. Scale bar: 50 μm. (E,G,I) Surviving neurons of each group were counted (n = 3); statistics by one-way ANOVA with Tukey’s multiple comparisons test; HE, F (2, 6) = 14.50, p = 0.0050; Nissl, F (2, 6) = 68.07, p < 0.0001; NeuN, F (2, 6) = 148.3, p < 0.0001. Data are depicted as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.