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. 2022 Nov 26;14(23):5836. doi: 10.3390/cancers14235836

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characteristics of MPM cell lines used for engraftment. (A) Summary of the ten MPM cell lines used in this study. Histological sub-type was defined by the supplying cell bank, tumour suppressor status was inferred from immunoblotting [48]; (B) MPM cell lines were transduced with lentiviral particles carrying pHIV-Luc-ZsGreen. Example fluorescence images of MESO-12T, MESO-8T and MSTO-211H cells grown in vitro show high efficiency of transduction (n = 1), other cell lines in Figure S1A; (C) Example in vitro analysis of luminescence signal shows proportionality with cell number for the MESO-8T cell line (n = 1), supporting kinetics data in Figure S1B.