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. 2022 Nov 26;14(23):5836. doi: 10.3390/cancers14235836

Figure 6.

Figure 6

MPM tumour nodules increase transcription of invasive and angiogenic genes compared to 2D cultures. (A) Representative image of MSTO-211H tumour nodule used for RNA extraction, scale bar 1 mm; (B,C) qRT-PCR analysis comparing expression of MMP9 (B) and VEGF (C) transcript levels in MSTO-211H grown as 2D in vitro cultures (n = 3) versus 3D CAM nodules (n = 6). Mean expression shown relative to the mean of ACTB and GAPDH (2∆Cq), error bars SD, unpaired t-test, * p < 0.05, ** p < 0.01. Supporting data for housekeeping genes in Figure S5. All experiments were in ovo.