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Optimization of LAMP–CRISPR detection system for Salmonella detection. (A) Fluorescence intensity using different concentrations (1, 2, 3, and 4 μM) of Cas12a; (B) Fluorescence intensity using different concentrations (0.5, 1.0, 1.5, 2.0, and 2.5 μM) of crRNA; (C) Fluorescence intensity using different concentrations (20, 40, 60, 80, and 100 μM) of report DNA; (D) Fluorescence intensity under different temperatures (25 °C, 31 °C, 37 °C, 43 °C, and 49 °C) of CRISPR/Cas12a system.
