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. 2022 Nov 26;11(23):3792. doi: 10.3390/cells11233792

Figure 6.

Figure 6

Endoplasmic reticulum stress makes a valuable contribution to the process of VSMC trans-differentiation, affected by ABCC6. (A) ERS-related proteins (XBP1s and p-EIF), immunohistochemistry, and immunofluorescence staining of non-MMD and MMD patients’ STA vessels, scale bar = 100 µm. (B) The correlation between intima ABCC6 expression level and intima thickness (r = 0.665, p = 0.0068), the correlation between intima XBP1s expression level and intima thickness (r = 0.611, p = 0.0155), and the correlation between ABCC6 expression level and XBP1s expression level in MMD patients’ intima (r = 0.8389, p < 0.0001), scale bar = 50 µm. (C) XBP1s and GRP78 protein of the VSMCs transferred with NC or ABCC6 siRNA (si) under non-OGD/OGD conditions were detected by Western blotting. The results of the CCK-8 assay (D) and Transwell assay (E) of the VSMCs transferred with the vector plasmid or the ABCC6 overexpression plasmid (OE) together, with or without 4-PBA, under non-OGD/OGD conditions, scale bar = 50 µm. (F) Western blot analysis of the protein expression of OPN and EREG in the VSMCs vector group, ABCC6 overexpression (OE) group, and the ABCC6 overexpression (OE) + 4PBA group under OGD conditions. The results of all groups are shown as mean ± SD. Two-way ANOVA and Tukey’s post hoc test was used. The significance level was accepted as * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.